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Methods and Concepts in the Life Sciences/DNA Sequencing; Usage on es.wikipedia.org Secuenciación Maxam-Gilbert; Usage on fa.wikipedia.org توالییابی به روش مکسام-گیلبرت; Usage on gl.wikipedia.org Secuenciación de Maxam e Gilbert; Usage on he.wikipedia.org ריצוף מקסאם-גילברט; Usage on ko.wikipedia.org
Maxam–Gilbert sequencing is a method of DNA sequencing developed by Allan Maxam and Walter Gilbert in 1976–1977. This method is based on nucleobase-specific partial chemical modification of DNA and subsequent cleavage of the DNA backbone at sites adjacent to the modified nucleotides. [1] An example Maxam–Gilbert sequencing reaction.
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The technique known as the “Plus and Minus” method, involved supplying all the components of the DNA but excluding the reaction of one of the four bases needed to complete the DNA. [44] In 1976, Gilbert and Maxam, invented a method for rapidly sequencing DNA while at Harvard, known as the Maxam–Gilbert sequencing. [45]
Walter Gilbert and Allan Maxam developed a DNA sequencing method - now called Maxam-Gilbert sequencing - which combined chemicals that cut DNA only at specific bases with radioactive labeling and polyacrylamide gel electrophoresis to determine the sequence of long DNA segments. [3] Allan Maxam and Walter Gilbert’s 1977 paper “A new method ...
The first DNA sequencing methods were developed by Gilbert (1973) [8] and Sanger (1975). [9] Gilbert introduced a sequencing method based on chemical modification of DNA followed by cleavage at specific bases whereas Sanger's technique is based on dideoxynucleotide chain termination. The Sanger method became popular due to its increased ...
The DNA template labeled at the 3' or 5' end, depending on the location of the binding site(s). Labels that can be used are: radioactivity and fluorescence. Radioactivity has been traditionally used to label DNA fragments for footprinting analysis, as the method was originally developed from the Maxam-Gilbert chemical sequencing technique.
Traditional DNA sequencing techniques such as Maxam-Gilbert or Sanger methods used polyacrylamide gels to separate DNA fragments differing by a single base-pair in length so the sequence could be read. Most modern DNA separation methods now use agarose gels, except for particularly small DNA fragments.