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LB medium bottle and LB agar plate Plate medium agar LB. Lysogeny broth (LB) is a nutritionally rich medium primarily used for the growth of bacteria. Its creator, Giuseppe Bertani, intended LB to stand for lysogeny broth, [1] but LB has also come to colloquially mean Luria broth, Lennox broth, life broth or Luria–Bertani medium. [2]
Microbial metabolism is the means by which a microbe obtains the energy and nutrients (e.g. carbon) it needs to live and reproduce.Microbes use many different types of metabolic strategies and species can often be differentiated from each other based on metabolic characteristics.
Bacteria are responsible for the process of nitrogen fixation, which is the conversion of atmospheric nitrogen into nitrogen-containing compounds (such as ammonia) that can be used by plants. Autotrophic bacteria derive their energy by making their own food through oxidation, like the Nitrobacter species, rather than feeding on plants or other ...
It is ideally spatially unstructured and temporally unstructured, in a steady state defined by the rates of nutrient supply and bacterial growth. In comparison to batch culture, bacteria are maintained in exponential growth phase, and the growth rate of the bacteria is known. Related devices include turbidostats and auxostats.
Thermophiles and hyperthermophiles employ different mechanisms to adapt their cells to heat, especially to the cell wall, plasma membrane, and its biomolecules (DNA, proteins, etc.): [12] The presence in their plasma membrane of long-chain and saturated fatty acids in bacteria and "ether" bonds (diether or tetraether) in archaea. In some ...
Acetivibrio thermocellus is an anaerobic, thermophilic bacterium. A. thermocellus has garnered research interest due to its cellulolytic and ethanologenic abilities, being capable of directly converting a cellulosic substrate into ethanol by consolidated bioprocessing.
Higher heat energy is required in a thermophilic system compared to a mesophilic system, but the thermophilic system requires much less time and has a larger gas output capacity and higher methane gas content, so one has to consider that trade-off carefully. [25] For solids content, low will handle up to 15% solid content.
This suggests that these sugars are building blocks within the cell that allow for the creation of the S-layer protecting Gram-positive bacteria. This connection to the S-layer is extremely important, because it is hypothesized that the S-layer is used to help protect the cell from the heat stress associated with hyperthermophilic environments.
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