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Immunohistochemistry or IHC staining of tissue sections (or immunocytochemistry, which is the staining of cells), is perhaps the most commonly applied immunostaining technique. [2] While the first cases of IHC staining used fluorescent dyes (see immunofluorescence ), other non-fluorescent methods using enzymes such as peroxidase (see ...
A Ziehl–Neelsen stain is an acid-fast stain used to stain species of Mycobacterium tuberculosis that do not stain with the standard laboratory staining procedures such as Gram staining. This stain is performed through the use of both red coloured carbol fuchsin that stains the bacteria and a counter stain such as methylene blue .
In pap smears, Light Green SF confers a blue staining for the cytoplasm of active cells such as columnar cells, parabasal squamous cells, and intermediate squamous cells. [3] It usually comes as a disodium salt. Its maximum absorption is at 630 (422) nm. The dye is not very durable — it has a tendency to fade.
Staining is often required to increase contrast, which prevents use on live cells in many situations. Bright-field illumination is useful for samples that have an intrinsic color, for example mitochondria or the observation of cytoplasmic streaming in Chara cells.
Acridine orange staining has to be performed at an acidic pH to obtain the differential staining, which allows bacterial cells to stain orange and tissue components to stain yellow or green. [ 8 ] Acridine orange is recorded as being used as a curing agent to cure selectable marker in antibiotic resistant organisms present in a sample.
DAPI can be used for fixed cell staining. The concentration of DAPI needed for live cell staining is generally very high; it is rarely used for live cells. [ 7 ] It is labeled non-toxic in its MSDS [ 8 ] and though it was not shown to have mutagenicity to E. coli , [ 9 ] it is labelled as a known mutagen in manufacturer information. [ 2 ]
Neutral red (toluylene red, Basic Red 5, or C.I. 50040) is a eurhodin dye used for staining in histology. It stains lysosomes red. [1] It is used as a general stain in histology, as a counterstain in combination with other dyes, and for many staining methods. Together with Janus Green B, it is used to stain embryonal tissues and supravital ...
RSCs and one RSC cell line (L1236 cells) but not other RSC cell lines express very high levels of ALOX15 (i.e., 15-lipoxygenase-1) or possibly ALOX15B (i.e. 15-lipoxygenase-2), enzymes that metabolize arachidonic acid and various other polyunsaturated fatty acids to a wide array of bioactive products including in particular those of the 15 ...