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An inoculation loop (also called a smear loop, inoculation wand or microstreaker) is a simple tool used mainly by microbiologists to pick up and transfer a small sample of microorganisms called inoculum from a microbial culture, e.g. for streaking on a culture plate. [1] [2] This process is called inoculation.
The inoculation loop is first sterilized by passing it through a flame. When the loop is cool, it is dipped into an inoculum such as a broth or patient specimen containing many species of bacteria. The inoculation loop is then dragged across the surface of the agar back and forth in a zigzag motion until approximately 30% of the plate has been ...
Sterilization of an inoculation needle via alcohol burner. The inoculation needle is sterilized using the aseptic technique. [1] [2] [3] [7] An open flame from an incinerator, a bunsen burner, or an alcohol burner is used to flame along the tip and the length of the needle that is to be in contact with the inoculum (or the propagule).
used for bacterial or fungal cultures Inoculation loop: used to inoculate test samples into culture media for bacterial or fungal cultures, antibiograms, etc. Sterilized by passing through a blue flame. Laminar flow cabinet: used to work aseptic Latex agglutination tiles: for serological analysis Lovibond comparator: a type of a colorimeter
The streak plate method is a way to physically separate the microbial population, and is done by spreading the inoculate back and forth with an inoculating loop over the solid agar plate. Upon incubation , colonies will arise and single cells will have been isolated from the biomass .
Flaming is done to inoculation loops and straight-wires in microbiology labs for streaking. Leaving the loop in the flame of a Bunsen burner or alcohol burner until it glows red ensures that any infectious agent is inactivated or killed. This is commonly used for small metal or glass objects, but not for large objects (see Incineration below ...
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Wet each disk with about four inoculating loops of deionized water. Use a loop to aseptically transfer a large mass of pure bacteria to the disk. Observe the disk for up to three minutes. If the area of inoculation turns dark-blue to maroon to almost black, then the result is positive.