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Incubators are essential for much experimental work in cell biology, microbiology and molecular biology and are used to culture both bacterial and eukaryotic cells. An incubator is made up of a chamber with a regulated temperature. Some incubators also regulate humidity, gas composition, or ventilation within that chamber.
A mesophile is an organism that grows best in moderate temperature, neither too hot nor too cold, with an optimum growth range from 20 to 45 °C (68 to 113 °F). [1] The optimum growth temperature for these organisms is 37 °C (about 99 °F). [2] The term is mainly applied to microorganisms.
Recently, a DNA polymerase derived from these bacteria, Bst polymerase, has become important in molecular biology applications. Bst polymerase has a helicase-like activity, making it able to unwind DNA strands. Its optimum functional temperature is between 60 and 65 °C and it is denatured at temperatures above 70 °C.
Bacterial culture of H. influenzae is performed on agar plates. The strongest growth is seen on chocolate agar at 37 °C in a CO 2-enriched incubator. [33] The ideal CO 2 concentration for the culture is ~5%. [34] However adequate growth is often seen on brain-heart infusion agar supplemented with hemin and nicotinamide adenine dinucleotide (NAD)
Coliform bacteria are defined as either motile or non-motile Gram-negative non-spore forming bacilli that possess β-galactosidase to produce acids and gases under their optimal growth temperature of 35–37 °C. [1] They can be aerobes or facultative aerobes, and are a commonly used indicator of low sanitary quality of foods, milk, and water. [2]
The optimal growth temperature range for B. cereus is 30-40 °C. ... Bacterial growth results in production of ... has an 8-to-16-hour incubation time, ...
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