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Ultraviolet-visible (UV-vis) spectroscopy involves energy levels that excite electronic transitions. Absorption of UV-vis light excites molecules that are in ground-states to their excited-states. [5] Visible region 400–700 nm spectrophotometry is used extensively in colorimetry science. It is a known fact that it operates best at the range ...
A UV-Vis spectrophotometer is an analytical instrument that measures the amount of ultraviolet (UV) and visible light that is absorbed by a sample. It is a widely used technique in chemistry, biochemistry, and other fields, to identify and quantify compounds in a variety of samples.
The vast majority of liquid chromatographic systems are equipped with ultraviolet (UV) absorption detectors. The most common UV-Vis detectors used are variable wavelength detectors (VWD), photo diode array detectors (PDA), and diode array detectors (DAD). [4] Variable wavelength detectors decide in advance which wavelength is needed for the ...
UV-visible spectroscopy. Add languages. Add links ... Upload file; Special pages; ... Get shortened URL; Download QR code; Print/export Download as PDF; Printable ...
DU Spectrophotometer, National Technical Laboratories, 1947. The DU spectrophotometer or Beckman DU, introduced in 1941, was the first commercially viable scientific instrument for measuring the amount of ultraviolet light absorbed by a substance.
With the aid of these rules the UV absorption maximum can be predicted, for example in these two compounds: [8] In the compound on the left, the base value is 214 nm (a heteroannular diene). This diene group has 4 alkyl substituents (labeled 1,2,3,4) and the double bond in one ring is exocyclic to the other (adding 5 nm for an exocyclic double ...
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In spectroscopy, a Soret peak or Soret band is an intense peak in the blue wavelength region of the visible spectrum. The peak is named after its discoverer, Jacques-Louis Soret . [ 1 ] The term is commonly used in absorption spectroscopy , corresponding to a wavelength of maximum absorption (electromagnetic radiation) ranging around 400 nm in ...