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Genes take up about 30% of the pufferfish genome and the coding DNA is about 10%. (Non-coding DNA = 90%.) The reduced size of the pufferfish genome is due to a reduction in the length of introns and less repetitive DNA. [8] [9] Utricularia gibba, a bladderwort plant, has a very small nuclear genome (100.7 Mb) compared to most plants.
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A conserved non-coding sequence (CNS) is a DNA sequence of noncoding DNA that is evolutionarily conserved. These sequences are of interest for their potential to regulate gene production. [1] CNSs in plants [2] and animals [1] are highly associated with transcription factor binding sites and other cis-acting regulatory elements.
Junk DNA (non-functional DNA) is a DNA sequence that has no known biological function. [ 1 ] [ 2 ] Most organisms have some junk DNA in their genomes —mostly, pseudogenes and fragments of transposons and viruses—but it is possible that some organisms have substantial amounts of junk DNA.
In the late 2000s, genome annotation shifted its attention towards identifying non-coding regions in DNA, which was achieved thanks to the appearance of methods to analyze transcription factor binding sites, DNA methylation sites, chromatin structure, and other RNA and regulatory region analysis techniques.
Eventually pseudogenes may be deleted from their genomes by chance of DNA replication or DNA repair errors, or they may accumulate so many mutational changes that they are no longer recognizable as former genes. Analysis of these degeneration events helps clarify the effects of non-selective processes in genomes.
A fact from Non-coding DNA appeared on Wikipedia's Main Page in the Did you know column on 21 March 2010 (check views). The text of the entry was as follows:
A non-coding RNA (ncRNA) is a functional RNA molecule that is not translated into a protein. The DNA sequence from which a functional non-coding RNA is transcribed is often called an RNA gene. Abundant and functionally important types of non-coding RNAs include transfer RNAs (tRNAs) and ribosomal RNAs (rRNAs), as well as small RNAs such as ...