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  2. Sticky and blunt ends - Wikipedia

    en.wikipedia.org/wiki/Sticky_and_blunt_ends

    The simplest DNA end of a double stranded molecule is called a blunt end. Blunt ends are also known as non-cohesive ends. In a blunt-ended molecule, both strands terminate in a base pair. Blunt ends are not always desired in biotechnology since when using a DNA ligase to join two molecules into one, the yield is significantly lower with blunt ...

  3. Integration host factor - Wikipedia

    en.wikipedia.org/wiki/Integration_host_factor

    When the Integration Host Factor was first discovered, it was only known for the site-specific recombination of bacteriophage. [4] This is all we knew for a while but through another article, we were able to find that with further research, IHF plays a key role in the scope of physiological processes of E. Coli, including site-specific recombination activities, phage packaging and partitioning ...

  4. Cosmid - Wikipedia

    en.wikipedia.org/wiki/Cosmid

    Those cells which did not take up the cosmid would be unable to grow. [3] Unlike plasmids, they can also be packaged in vitro into phage capsids, a step which requires cohesive ends, also known as cos sites also used in cloning with a lambda phage as a vector, however nearly all the lambda genes have been deleted with the exception of the cos ...

  5. Mobile genetic elements - Wikipedia

    en.wikipedia.org/wiki/Mobile_genetic_elements

    DNA transposons, LTR retrotransposons, SINEs, and LINEs make up a majority of the human genome. Mobile genetic elements (MGEs), sometimes called selfish genetic elements, [1] are a type of genetic material that can move around within a genome, or that can be transferred from one species or replicon to another. MGEs are found in all organisms.

  6. Ligation (molecular biology) - Wikipedia

    en.wikipedia.org/wiki/Ligation_(molecular_biology)

    The mechanism of the ligation reaction was first elucidated in the laboratory of I. Robert Lehman. [4] [5] Two fragments of DNA may be joined by DNA ligase which catalyzes the formation of a phosphodiester bond between the 3'-hydroxyl group (-OH) at one end of a strand of DNA and the 5'-phosphate group (-PO4) of another.

  7. Peppermint Meltaways Are About To Be Your New Holiday Go-To ...

    www.aol.com/peppermint-meltaways-holiday-cookies...

    Preheat oven to 350° and line a large baking sheet with parchment. In a food processor, pulse flour, powdered sugar, cornstarch, baking powder, and salt a few times to combine.

  8. Health benefits of rhubarb and how to uniquely include the ...

    www.aol.com/health-benefits-rhubarb-uniquely...

    Rhubarb is a vegetable high in fiber. "[Rhubarb is] rich in fiber, so it really helps with digestion. [It] has a pretty good source of fiber per serving," Wright told Fox News Digital.

  9. BamHI - Wikipedia

    en.wikipedia.org/wiki/BamHI

    BamHI, like other type II restriction endonucleases, often requires divalent metals as cofactors to catalyze DNA cleavage. [2] Two-metal ion mechanism is one of the possible catalytic mechanisms of BamHI since the BamHI crystal structure has the ability to bind two metal ions at the active site, which is suitable for the classical two-metal ion mechanism to proceed.