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Incubators are essential for much experimental work in cell biology, microbiology and molecular biology and are used to culture both bacterial and eukaryotic cells. An incubator is made up of a chamber with a regulated temperature. Some incubators also regulate humidity, gas composition, or ventilation within that chamber.
A mesophile is an organism that grows best in moderate temperature, neither too hot nor too cold, with an optimum growth range from 20 to 45 °C (68 to 113 °F). [1] The optimum growth temperature for these organisms is 37 °C (about 99 °F). [2] The term is mainly applied to microorganisms.
The phase-change incubator is a low-cost, low-maintenance incubator that tests for microorganisms in water supplies. It uses small balls containing a chemical compound that, when heated and then kept insulated, will stay at 37 °C (approx. 99 °F) for 24 hours.
A microbiological culture, or microbial culture, is a method of multiplying microbial organisms by letting them reproduce in predetermined culture medium under controlled laboratory conditions. Microbial cultures are foundational and basic diagnostic methods used as research tools in molecular biology .
Most water baths have a digital or an analogue interface to allow users to set a desired temperature, but some water baths have their temperature controlled by a current passing through a reader. Uses include warming of reagents , melting of substrates , determination of boiling point , or incubation of cell cultures.
Geobacillus stearothermophilus (previously Bacillus stearothermophilus) [1] [2] is a rod-shaped, Gram-positive bacterium and a member of the phylum Bacillota.The bacterium is a thermophile and is widely distributed in soil, hot springs, ocean sediment, and is a cause of spoilage in food products.
The dilution plates are then incubated at a temperature of 37 degrees Celsius. [4] The plates are then incubated for sixteen to eighteen hours, although incubation time may be less for bacteria populations that divide quickly. [1]: 374 After incubation, the plates are examined to determine if bacterial growth has occurred in the inoculated ...
The molten agar is cooled to about 45 degrees Celsius and is poured using a sterile method into a petri dish containing a specific diluted sample. From here, the plates are rotated to ensure the samples are uniformly mixing with the agar. Incubation of the plates is the next step and is carried out for about 3 days at 20 to 30 degrees Celsius.
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