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Massive parallel sequencing or massively parallel sequencing is any of several high-throughput approaches to DNA sequencing using the concept of massively parallel processing; it is also called next-generation sequencing (NGS) or second-generation sequencing.
Several new methods for DNA sequencing were developed in the mid to late 1990s and were implemented in commercial DNA sequencers by 2000. Together these were called the "next-generation" or "second-generation" sequencing (NGS) methods, in order to distinguish them from the earlier methods, including Sanger sequencing. In contrast to the first ...
ChiRP-Seq is one of these new methods which uses the massively parallel sequencing capability of 2nd generation sequencers to catalog the binding sites of these novel RNA molecules on a genome. Although many have believed that RNAs mainly encode for proteins a very large portion of the eukaryotic genome is composed of RNAs that do not.
These two methods were used to put together our human reference genome. However, since Sanger sequencing is low throughput and expensive, only a few genomes are assembled with Sanger sequencing. Second-generation sequencing reads are short, and these sequencing techniques can efficiently and cost-effectively sequence hundreds of millions of reads.
The workflow of a typical hybrid genome assembly experiment using second- and third-generation sequencing technologies. Figure adapted from Wang et al., 2012 [14]. One hybrid approach to genome assembly involves supplementing short, accurate second-generation sequencing data (i.e. from IonTorrent, Illumina or Roche 454) with long less accurate third-generation sequencing data (i.e. from PacBio ...
2 Base Encoding, also called SOLiD (sequencing by oligonucleotide ligation and detection), is a next-generation sequencing technology developed by Applied Biosystems and has been commercially available since 2008. These technologies generate hundreds of thousands of small sequence reads at one time.
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Polony sequencing is a development of the polony technology from the late 1990s and 2000s. [5] Methods were developed in 2003 to sequence in situ polonies using single-base extension which could achieve 5-6 bases reads. [6] By 2005, these early attempts had been overhauled to develop the existing polony sequencing technology. [7]
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