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A volcano plot is constructed by plotting the negative logarithm of the p value on the y axis (usually base 10). This results in data points with low p values (highly significant) appearing toward the top of the plot. The x axis is the logarithm of the fold change between the two conditions. The logarithm of the fold change is used so that ...
RNA-Seq can also be used to determine exon/intron boundaries and verify or amend previously annotated 5' and 3' gene boundaries. Recent advances in RNA-Seq include single cell sequencing, bulk RNA sequencing, [6] 3' mRNA-sequencing, in situ sequencing of fixed tissue, and native RNA molecule sequencing with single-molecule real-time sequencing. [7]
Heatmap of RNA-Seq data showing two dendrograms in the left and top margins. A dendrogram is a diagram representing a tree. This diagrammatic representation is frequently used in different contexts: in hierarchical clustering, it illustrates the arrangement of the clusters produced by the corresponding analyses. [4]
In bioinformatics, sequence analysis is the process of subjecting a DNA, RNA or peptide sequence to any of a wide range of analytical methods to understand its features, function, structure, or evolution. It can be performed on the entire genome, transcriptome or proteome of an organism, and can also involve only selected segments or regions ...
RNA-Seq [1] [2] [3] is a technique [4] that allows transcriptome studies (see also Transcriptomics technologies) based on next-generation sequencing technologies. This technique is largely dependent on bioinformatics tools developed to support the different steps of the process. Here are listed some of the principal tools commonly employed and ...
Trajectory inference as implemented in Slingshot for (a) a simulated two-dimensional dataset and (b) a single-cell RNA-seq dataset of the olfactory epithelium.. Trajectory inference or pseudotemporal ordering is a computational technique used in single-cell transcriptomics to determine the pattern of a dynamic process experienced by cells and then arrange cells based on their progression ...
The three dimensional structure of biological macromolecules like proteins and nucleic acids play a critical role in determining their functional role. [2] This process of decoding function from the sequence is an experimentally and computationally challenging question addressed widely.
Volcano plot may refer to: . Sabatier principle - a concept in chemical catalysis that relates the optimal concentrations of catalysts and substrates; Volcano plot (statistics) - a type of graph used to relate fold-change to p-value that is commonly used in genomics and other omic experiments involving thousands of data-points
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