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A calibration curve plot showing limit of detection (LOD), limit of quantification (LOQ), dynamic range, and limit of linearity (LOL).. In analytical chemistry, a calibration curve, also known as a standard curve, is a general method for determining the concentration of a substance in an unknown sample by comparing the unknown to a set of standard samples of known concentration. [1]
The microscope can then use the change in resonant frequency (f) as the SPM reference channel, either in feedback mode, or it can be recorded directly in constant height mode. While recording frequency-modulated images, an additional feedback loop is normally used to keep the amplitude of resonance constant, by adjusting the drive amplitude.
The appearance of markers in images may act as a reference for image scaling, or may allow the image and physical object, or multiple independent images, to be correlated. By placing fiducial markers at known locations in a subject, the relative scale in the produced image may be determined by comparison of the locations of the markers in the ...
Scanning electron microscope image of pollen (false colors) Microscopic examination in a biochemical laboratory. Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). [1]
Electron-beam lithography systems used in commercial applications are dedicated e-beam writing systems that are very expensive (> US$1M). For research applications, it is very common to convert an electron microscope into an electron beam lithography system using relatively low cost accessories (< US$100K). Such converted systems have produced ...
The exit wave now passes through the imaging system of the microscope where it undergoes further phase change and interferes as the image wave in the imaging plane (mostly a digital pixel detector like a CCD camera). The recorded image is not a direct representation of the samples crystallographic structure. For instance, high intensity might ...
By 1978, the first theoretical ideas had been developed to break the Abbe limit, which called for using a 4Pi microscope as a confocal laser-scanning fluorescence microscope where the light is focused from all sides to a common focus that is used to scan the object by 'point-by-point' excitation combined with 'point-by-point' detection. [14]
At this point the master image could be arrayed into a multi-chip image called a reticle. The reticle was originally a 10X larger image of a single chip. The reticle was by step-and-repeater photolithography and etching used to produce a photomask with image-size the same as the final chip.