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Radial arrangement of PP-technique (3 or more channels) provides the possibility to compare chemotactic activity of different cell populations or study preference between ligands. [ 1 ] Counting of cells: positive responder cells could be counted from the front of migrating cells, after staining or in native conditions in light microscope.
A finned air cooled heatsink with fan clipped onto a CPU, with a smaller passive heatsink without fan in the background A 3-fan heatsink mounted on a video card to maximize cooling efficiency of the GPU and surrounding components Commodore 128DCR computer's switch-mode power supply, with a user-installed 60 mm cooling fan.
The nonlinear nature of three photon microscopy confines the excitation target to a smaller volume, reducing out-of-focus light as well as minimizing photobleaching on the biological sample. [16] These advantages of three-photon microscopy gives it an edge in visualize in vivo and ex vivo tissue morphology and physiology at a cellular level ...
This requires an existing defect in the crystal, as is done by radiation, and is accelerated by heat, high current density and emitted light. With LEDs, gallium arsenide and aluminium gallium arsenide are more susceptible to this than gallium arsenide phosphide and indium phosphide ; gallium nitride and indium gallium nitride are insensitive to ...
A two-photon microscope is also a laser-scanning microscope, but instead of UV, blue or green laser light, a pulsed infrared laser is used for excitation. Only in the tiny focus of the laser is the intensity high enough to generate fluorescence by two-photon excitation , which means that no out-of-focus fluorescence is generated, and no pinhole ...
Lattice light-sheet microscopy is a modified version of light sheet fluorescence microscopy that increases image acquisition speed while decreasing damage to cells caused by phototoxicity. This is achieved by using a structured light sheet to excite fluorescence in successive planes of a specimen, generating a time series of 3D images which can ...
AOL
Light field microscopy (LFM) is a scanning-free 3-dimensional (3D) microscopic imaging method based on the theory of light field.This technique allows sub-second (~10 Hz) large volumetric imaging ([~0.1 to 1 mm] 3) with ~1 μm spatial resolution in the condition of weak scattering and semi-transparence, which has never been achieved by other methods.