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A quarter of the world's SARS-CoV-2 viral genomes were sequenced with nanopore devices. The technology offers an important tool for combating antimicrobial resistance. [11] In 2020, China-based Qitan Technology launched its nanopore single-molecule gene sequencer, [12] while in 2024 MGI Tech launched its own products. [13]
A nanopore is a pore of nanometer size. It may, for example, be created by a pore-forming protein or as a hole in synthetic materials such as silicon or graphene. When a nanopore is present in an electrically insulating membrane, it can be used as a single-molecule detector.
Pore-C workflow. Many methods to characterize the 3D genome are variations on 3C technology. [5] Like other 3C-based technologies, [5] Pore-C seeks to characterize the architecture of the 3D genome by determining which genomic loci are in close spatial proximity (within ~200 nm). [2]
Sequencing technologies with a different approach than second-generation platforms were first described as "third-generation" in 2008–2009. [4]There are several companies currently at the heart of third generation sequencing technology development, namely, Pacific Biosciences, Oxford Nanopore Technology, Quantapore (CA-USA), and Stratos (WA-USA).
Other technologies have emerged, including Nanopore technology. Though the sequencing accuracy of Nanopore technology is lower than those above, its read length is on average much longer. [35] This generation of long reads is valuable especially in de novo whole-genome sequencing applications. [36]
MLST is highly unambiguous and portable. Materials required for ST determination can be exchanged between laboratories. Primer sequences and protocols can be accessed electronically. It is reproducible and scalable. MLST is automated, combines advances in high throughput sequencing and bioinformatics with established population genetics techniques.
Oxford Nanopore Technologies plc is a UK-based company which develops and sells nanopore sequencing products (including the portable DNA sequencer, MinION) for the direct, electronic analysis of single molecules. [2] [3] [4] It is listed on the London Stock Exchange and is a constituent of the FTSE 250 Index. [5]
A nanopore battery is a rechargeable battery that is a composite of billions of nanoscale batteries formed within the pores of a substrate. [1] The space inside the holes is so small that billions of pores combined equal the volume of a grain of sand. Each pore's diameter was some one eighty-thousandth the width of a human hair. [1]