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Isopropyl β-d-1-thiogalactopyranoside (IPTG) is a molecular biology reagent. This compound is a molecular mimic of allolactose , a lactose metabolite that triggers transcription of the lac operon , and it is therefore used to induce protein expression where the gene is under the control of the lac operator .
In E. coli BL21(DE3) the expression of the T7-RNAP is suppressed by the constitutively expressed LacI repressor. LacI binds the lac operator, which is located downstream of the LacUV5 promoter, preventing the production of the T7-RNAP. However, upon supplementation of IPTG, the LacI repressor dissociates from the lac operator, allowing for the ...
IPTG is a reagent which mimics the structure of allolactose, and can therefore bind to the lac repressor and prevent it from inhibiting gene expression. Once enough IPTG is added, the T7 gene is normally transcribed and so transcription of the gene of interest downstream of the T7 promoter also begins. [6]
Miller and Sweatt demonstrated that rats trained in a contextual fear conditioning paradigm had elevated levels of mRNA for DNMT3a and DNMT3b in the hippocampus. [4] Fear conditioning is an associative memory task where a context, like a room, is paired with an aversive stimulus, like a foot shock; animals who have learned the association show higher levels of freezing behavior when exposed to ...
Metaplasticity is a term describing the likelihood that a given stimulus will induce neuronal plasticity, based on the previous activity experienced by that neuron. Several studies provide evidence that neurons receiving “priming activity” (such as neurotransmitters, paracrine signals, or hormones) minutes to days prior will show a lower threshold for induction of long term potentiation (LTP).
Teams work on the uncovered mass grave, believed to contain the remains of civilians killed by the ousted Assad regime, on the road to Damascus International Airport in the Syrian capital Damascus ...
LTP could be produced by repetitive stimulation of the presynaptic terminals, and it is believed to play a role in memory function in the hippocampus, amygdala and other cortical brain structures in mammals. [2] [3] Long-term potentiation occurs when synaptic transmission becomes more effective as a result of recent activity.
A person was arrested after attempting to climb a temporary fence near the White House this morning, according to a U.S. Secret Service spokesperson. The Secret Service said the person was ...