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In molecular biology, PVP can be used as a blocking agent during Southern blot analysis as a component of Denhardt's buffer. It is also exceptionally good at absorbing polyphenols during DNA purification. Polyphenols are common in many plant tissues and can deactivate proteins if not removed and therefore inhibit many downstream reactions like PCR.
Polyvinylpolypyrrolidone (polyvinyl polypyrrolidone, PVPP, crospovidone, crospolividone, or E1202) is a highly cross-linked modification of polyvinylpyrrolidone (PVP). The cross-linked form of PVP is used as a disintegrant (see also excipients ) in pharmaceutical tablets. [ 1 ]
It is produced industrially by vinylation of 2-pyrrolidone, i.e. the base-catalyzed reaction with acetylene. [2] It is the precursor to polyvinylpyrrolidone (PVP), an important synthetic material. The NVP monomer is commonly used as a reactive diluent in ultraviolet and electron - beam curable polymers applied as inks , coatings or adhesives .
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Due to the electron-withdrawing effect of the ring nitrogen atom, 2-vinylpyridine adds nucleophiles such as methoxide, cyanide, hydrogen sulfide at the vinylic site to give addition products. The addition product of methanol to 2-vinylpyridine, 2-(2-methoxyethyl)pyridine is a veterinary anthelmintic .
2-Pyrrolidone, also known as 2-pyrrolidinone or butyrolactam, is an organic compound consisting of a 5-membered lactam, making it the simplest γ-lactam. It is a colorless liquid that is miscible with water and most common organic solvents.
4-Vinylpyridine (4-VP) is an organic compound with the formula CH 2 CHC 5 H 4 N. It is a derivative of pyridine with a vinyl group in the 4-position. It is a colorless liquid, although impure samples are often brown.
Films of PPy are yellow but darken in the air due to some oxidation. Doped films are blue or black depending on the degree of polymerization and film thickness.