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A UV-Vis spectrophotometer is an analytical instrument that measures the amount of ultraviolet (UV) and visible light that is absorbed by a sample. It is a widely used technique in chemistry, biochemistry, and other fields, to identify and quantify compounds in a variety of samples.
As can be seen in Figure 1, these detectors have a light source, a dispersion element that is a diffraction grating or prism, a flow cell, to where the sample arrives directly from the chromatographic column, an optical bench of lenses and mirrors, and a diode that receives the light coming from the optical system and translates it into a ...
A two-photon microscope is also a laser-scanning microscope, but instead of UV, blue or green laser light, a pulsed infrared laser is used for excitation. Only in the tiny focus of the laser is the intensity high enough to generate fluorescence by two-photon excitation , which means that no out-of-focus fluorescence is generated, and no pinhole ...
An extremely intense pulse of short-wave UV light in a flashtube produced this blue persistent-phosphorescence in the amorphous, fused silica envelope, lasting as long as 20 minutes after the 3.5 microsecond flash. An electron microscope reveals vacancy defects in a crystalline lattice of molybdenum disulfide.
The microscope setup is based on an inverted microscope design. [ 2 ] [ 3 ] [ 4 ] An automated stage is used to record larger areas by mosaicing a series of single adjacent frames. The LED light is focused using a ball lens with a short focal length onto the sample surface in an oblique-angle cis-illumination scheme since standard microscopy ...
If the particle has a higher refractive index than the liquid surrounding it then it behaves as a convex lens and focuses a parallel beam of light on the side opposite the source of the light. Looking through the microscope this is seen as a bright ring of light, the Becke` Line, moving in from the edge as the particle is dropped out of focus ...
Quartz or mirror-based optics can be used for studying samples from the ultraviolet (UV), down to 200 nm, to the near infrared (NIR) up to 2100 nm. Samples that emit electromagnetic radiation via fluorescence, phosphorescence or photoluminescence when exposed to light, can be quantitatively investigated using a variety of excitation and barrier ...
Köhler illumination is a method of specimen illumination used for transmitted and reflected light (trans- and epi-illuminated) optical microscopy.Köhler illumination acts to generate an even illumination of the sample and ensures that an image of the illumination source (for example a halogen lamp filament) is not visible in the resulting image.
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