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Resolution in the context of structural biology is the ability to distinguish the presence or absence of atoms or groups of atoms in a biomolecular structure. Usually, the structure originates from methods such as X-ray crystallography, electron crystallography, or cryo-electron microscopy. The resolution is measured of the "map" of the ...
Microscopy. Scanning electron microscope image of pollen (false colors) Microscopic examination in a biochemical laboratory. Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). [1]
Aspect ratio. The aspect ratio of a geometric shape is the ratio of its sizes in different dimensions. For example, the aspect ratio of a rectangle is the ratio of its longer side to its shorter side—the ratio of width to height, [1][2] when the rectangle is oriented as a "landscape". The aspect ratio is most often expressed as two integer ...
Fluorescence and confocal microscopes operating principle. Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. [1]
The subsampling scheme is commonly expressed as a three-part ratio J:a:b (e.g. 4:2:2) or four parts, if alpha channel is present (e.g. 4:2:2:4), that describe the number of luminance and chrominance samples in a conceptual region that is J pixels wide and 2 pixels high. The parts are (in their respective order):
Aspect ratio (image) The aspect ratio of an image is the ratio of its width to its height. It is expressed as two numbers separated by a colon, width:height. Common aspect ratios are 1.85:1 and 2.40:1 in cinematography, 4:3 and 16:9 in television, and 3:2 in still photography.
The 1280 × 1024 resolution is not the standard 4:3 aspect ratio, instead it is a 5:4 aspect ratio (1.25:1 instead of 1. 3:1). A standard 4:3 monitor using this resolution will have rectangular rather than square pixels, meaning that unless the software compensates for this the picture will be distorted, causing circles to appear elliptical.
Live-cell imaging is the study of living cells using time-lapse microscopy. It is used by scientists to obtain a better understanding of biological function through the study of cellular dynamics. [1] Live-cell imaging was pioneered in the first decade of the 21st century. One of the first time-lapse microcinematographic films of cells ever ...