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pICalculax — Isoelectric point (pI) predictor for chemically modified peptides and proteins; SWISS-2DPAGE Archived 2016-12-10 at the Wayback Machine — a database of isoelectric points coming from two-dimensional polyacrylamide gel electrophoresis (~ 2,000 proteins) PIP-DB — a Protein Isoelectric Point database (~ 5,000 proteins)
The isoelectric point is the pH at which a compound - in this case a protein - has no net charge. A protein's isoelectric point or PI can be determined using the pKa of the side chains, if the amino (positive chain) is able to cancel out the carboxyl (negative) chain, the protein would be at its PI.
Isoelectric focusing (IEF), also known as electrofocusing, is a technique for separating different molecules by differences in their isoelectric point (pI). [ 1 ] [ 2 ] It is a type of zone electrophoresis usually performed on proteins in a gel that takes advantage of the fact that overall charge on the molecule of interest is a function of the ...
Capillary electrophoresis (CE) is a family of electrokinetic separation methods performed in submillimeter diameter capillaries and in micro- and nanofluidic channels.Very often, CE refers to capillary zone electrophoresis (CZE), but other electrophoretic techniques including capillary gel electrophoresis (CGE), capillary isoelectric focusing (CIEF), capillary isotachophoresis and micellar ...
Isoelectric may refer to: Isoelectric point, the pH at which a particular molecule carries no net electrical charge; Isoelectric focusing, a technique for separating different molecules by differences in their isoelectric point; Isoelectric line representing the absence of electrical activity on an electrocardiogram
A fixture at any fast food restaurant or backyard barbecue is American cheese. These orange, plastic-wrapped slices are unparalleled in terms of meltability. For many, when it comes to making a ...
Previous real estate agents recommended converting the space to a garage for a classic car collection. The previous owners attempted to fashion the shelter into a Tier IV data center — the ...
Leucine (symbol Leu or L) [3] is an essential amino acid that is used in the biosynthesis of proteins.Leucine is an α-amino acid, meaning it contains an α-amino group (which is in the protonated −NH 3 + form under biological conditions), an α-carboxylic acid group (which is in the deprotonated −COO − form under biological conditions), and a side chain isobutyl group, making it a non ...