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A diamond knife blade used for cutting ultrathin sections (typically 70 to 350 nm) for transmission electron microscopy The cutting edge of a disposable blade for a microtome under a microscope The selection of microtome knife blade profile depends upon the material and preparation of the samples, as well as the final sample requirements (e.g ...
Ultramicrotomy is a method for cutting specimens into extremely thin slices, called ultra-thin sections, that can be studied and documented at different magnifications in a transmission electron microscope (TEM). It is used mostly for biological specimens, but sections of plastics and soft metals can also be prepared.
A bright-field microscope has many important parts including; the condenser, the objective lens, the ocular lens, the diaphragm, and the aperture. Some other pieces of the microscope that are commonly known are the arm, the head, the illuminator, the base, the stage, the adjusters, and the brightness adjuster.
The single lens with its attachments, or the system of lenses and imaging equipment, along with the appropriate lighting equipment, sample stage, and support, makes up the basic light microscope. The most recent development is the digital microscope, which uses a CCD camera to focus on the exhibit of interest. The image is shown on a computer ...
Scanning electron micrograph of a nanoknife. An individual carbon nanotube is being stretched between two tungsten needles. The triangular tip is an atomic force cantilever, measuring the knife's breaking point.( A nanoknife is a carbon nanotube-based prototype compression cutting tool intended for sectioning of biological cells. [1]
The Knife-Edge Scanning Microscope (KESM) was invented and patented in the late 1990s by Bruce McCormick at Texas A&M University. [1] The microscope is intended to produce high-resolution data sets in order to reconstruct 3D cellular structures .
Dark-field microscopy is a very simple yet effective technique and well suited for uses involving live and unstained biological samples, such as a smear from a tissue culture or individual, water-borne, single-celled organisms. [4] Considering the simplicity of the setup, the quality of images obtained from this technique is impressive.
Köhler illumination is a method of specimen illumination used for transmitted and reflected light (trans- and epi-illuminated) optical microscopy.Köhler illumination acts to generate an even illumination of the sample and ensures that an image of the illumination source (for example a halogen lamp filament) is not visible in the resulting image.