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A high-performance countercurrent chromatography system. Countercurrent chromatography (CCC, also counter-current chromatography) is a form of liquid–liquid chromatography that uses a liquid stationary phase that is held in place by inertia of the molecules composing the stationary phase accelerating toward the center of a centrifuge due to centripetal force [1] and is used to separate ...
Droplet countercurrent chromatography (DCCC or DCC) was introduced in 1970 by Tanimura, Pisano, Ito, and Bowman. [1] DCCC is considered to be a form of liquid-liquid separation, which includes countercurrent distribution and countercurrent chromatography, that employs a liquid stationary phase held in a collection of vertical glass columns connected in series.
Periodic counter-current chromatography (PCC) is a method for running affinity chromatography in a quasi-continuous manner. Today, the process is mainly employed for the purification of antibodies in the biopharmaceutical industry [ 1 ] as well as in research and development.
The Ho Chi Minh City University of Technology (HCMUT; Vietnamese: Trường Đại học Bách khoa, Đại học Quốc gia Thành phố Hồ Chí Minh, lit. 'Polytechnic of Vietnam National University, Ho Chi Minh City') [1] is a research university in Ho Chi Minh City, Vietnam. HCMUT is a member of Vietnam National University, Ho Chi Minh ...
All chromatographic purifications and separations which are executed via solvent gradient batch chromatography can be performed using MCSGP. Typical examples are reversed phase purification of peptides, hydrophobic interaction chromatography for fatty acids or for example ion exchange chromatography of proteins or antibodies. The process can ...
Countercurrent distribution, therefore, is a method of using a series of vessels (separatory funnels) to separate compounds by a sequence of liquid-liquid extraction operations. Contrary to liquid-liquid extraction, in the CCD instruments the upper phase is decanted from the lower phase once the phases have settled.
Le Quy Don Technical University (Vietnamese: Đại học Kỹ thuật Lê Quý Đôn), also known as Military Technical Academy (Học viện Kỹ thuật Quân sự), [1] was founded in 1966 and is one of the national key universities in Vietnam. Le Quy Don Technical University has developed into an open, multidisciplinary, research-oriented ...
Countercurrent chromatography usually uses a planetary gear motion without rotary seals, while centrifugal partition chromatography uses circular rotation with rotary seals for liquid connection. CCC has interchanging mixing and settling zones in the coil tube, so atomization, extraction and settling are time and zone separated.