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An MSA plate with Micrococcus sp. (1), Staphylococcus epidermidis (2) and S. aureus colonies (3). Mannitol salt agar or MSA is a commonly used selective and differential growth medium in microbiology. It encourages the growth of a group of certain bacteria while inhibiting the growth of others. [1]
Staphylococcus aureus (/ ˌ s t æ f ɪ l ə ˈ k ɒ k ə s ˈ ɔːr i ə s,-l oʊ-/, [16] [17] Greek σταφυλόκοκκος ' grape-cluster berry ', Latin aureus, ' golden ') is a facultative anaerobic, gram-positive coccal (round) bacterium also known as "golden staph" and "oro staphira".
Resistance to antibiotics in S. aureus can be quantified by determining the amount of the antibiotic that must be used to inhibit growth. If S. aureus is inhibited at a concentration of vancomycin less than or equal to 4 μg/ml, it is said to be susceptible. If a concentration greater than 32 μg/ml is necessary to inhibit growth, it is said to ...
It is used to isolate Staphylococcus aureus from clinical specimens and food. It was first described by Vogel and Johnson, who modified the Tellurite Glycine Agar recipe by Zebovitz et al. by doubling the mannitol concentration to 1% (w/v) and adding Phenol red as a pH indicator. [1] It is widely available commercially.
The hemolysis of some weakly beta-hemolytic organisms is enhanced when streaked close to certain beta hemolytic strains of Staphylococcus aureus. This phenomenon is the mechanism behind the CAMP test , [ 2 ] a test that was historically used for the identification of Streptococcus agalactiae and Listeria monocytogenes . [ 3 ]
Staphylococcus growth in Baird-Parker (BP) agar.. Baird-Parker agar is a type of agar used for the selective isolation of gram-positive Staphylococci species. [1] It contains lithium chloride and tellurite to inhibit the growth of alternative microbial flora, while the included pyruvate and glycine promote the growth of Staphylococci. [1]
S.aureus produces two forms of coagulase (i.e., bound coagulase and free coagulase). Bound coagulase, otherwise known as "clumping factor", can be detected by carrying out a slide coagulase test, and free coagulase can be detected using a tube coagulase test.
For example, if a microbiologist observes colonies that resemble a Staphylococcus species, they may perform a catalase test to confirm that it belongs to the genus Staphylococcus, and a coagulase test to determine whether it is a coagulase-negative staphylococcus or a more pathogenic species, such as S. aureus. [3]: 101 [8]: 203