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Culturomics is the high-throughput cell culture of bacteria that aims to comprehensively identify strains or species in samples obtained from tissues such as the human gut or from the environment. [ 1 ] [ 2 ] This approach was conceived as an alternative, complementary method to metagenomics , which relies on the presence of homologous ...
Microbial cultures on solid and liquid media. A microbiological culture, or microbial culture, is a method of multiplying microbial organisms by letting them reproduce in predetermined culture medium under controlled laboratory conditions. Microbial cultures are foundational and basic diagnostic methods used as research tools in molecular biology.
National Collection of Type Cultures (NCTC) is one of the four culture collections that constitutes the Culture Collections operated by the UK Health Security Agency. It is a non-profit culture collection repository located in the UK. NCTC maintains over 5100 bacterial cultures, over 100 Mycoplasmas and more than 500 plasmids, host strains ...
Microbial food cultures are live bacteria, yeasts or moulds used in food production. Microbial food cultures carry out the fermentation process in foodstuffs. Used by humans since the Neolithic period (around 10 000 years BC) [1] fermentation helps to preserve perishable foods and to improve their nutritional and organoleptic qualities (in this case, taste, sight, smell, touch).
The consortium comprises more than 269,000 publicly available strains of bacteria including mycobacteria and cyanobacteria, filamentous fungi, yeasts, diatoms and plasmids. BCCM is embedded in international initiatives such as the World Federation of Culture Collections and operates in compliance with the rules of the Nagoya Protocol.
About half of the dry mass of a bacterial cell consists of carbon, and also about half of it can be attributed to proteins. Therefore, a typical fully grown 1-liter culture of Escherichia coli (at an optical density of 1.0, corresponding to c. 10 9 cells/ml) yields about 1 g wet cell mass. [2]
In 2012, the freely accessible database BacDive (The Bacterial Diversity Metadatabase) [10] was established, which is maintained and curated by the DSMZ. The database contains information on a wide variety of strains of prokaryotes ; in 2016, information on 53,978 strains could be found there, [ 11 ] and by 2021, the number had increased to ...
Gram-negative bacteria will stain a pink color due to the thin layer of peptidoglycan. If a bacteria stains purple, due to the thick layer of peptidoglycan, the bacteria is a gram-positive bacteria. [4] In clinical microbiology numerous other staining techniques for particular organisms are used (acid fast bacterial stain for mycobacteria).