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This process of the second bacterial cell taking up new genetic material is called transformation. In molecular biology and genetics, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material from its surroundings through the cell membrane(s).
Bacterial recombination is a type of genetic recombination in bacteria characterized by DNA transfer from one organism called donor to another organism as recipient. This process occurs in three main ways: Transformation, the uptake of exogenous DNA from the surrounding environment. Transduction, the virus-mediated transfer of DNA between bacteria.
Griffith's experiment discovering the "transforming principle" in Streptococcus pneumoniae (pneumococcal) bacteria. Griffith's experiment, [1] performed by Frederick Griffith and reported in 1928, [2] was the first experiment suggesting that bacteria are capable of transferring genetic information through a process known as transformation.
In bacteria, transformation is a process of gene transfer that ordinarily occurs between individual cells of the same bacterial species. Transformation involves integration of donor DNA into the recipient chromosome by recombination. This process appears to be an adaptation for repairing DNA damages in the recipient chromosome by HRR. [13]
Transformation in bacteria happens in a certain environment. Natural transformation is a bacterial adaptation for DNA transfer (HGT) that depends on the expression of numerous bacterial genes whose products are responsible for this process. [72] [73] In general
Transfection is the process of deliberately introducing naked or purified nucleic acids into eukaryotic cells. [1] [2] It may also refer to other methods and cell types, although other terms are often preferred: "transformation" is typically used to describe non-viral DNA transfer in bacteria and non-animal eukaryotic cells, including plant cells.
[3] [8] Transformation is a naturally occurring process in bacteria. To successfully take up exogenous DNA, bacteria need to be in a state of competence. Some bacteria are naturally competent, but usually only for a brief time at a certain stage of their growth cycle. [9] Bacteria can also be made competent through a variety of chemical treatments.
Natural bacterial transformation involves the transfer of DNA from a donor bacterium to a recipient bacterium, where both donor and recipient are ordinarily of the same species. Transformation, unlike bacterial conjugation and transduction, depends on numerous bacterial gene products that specifically interact to perform this process. [84]