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Replication cycle of L-A helper, shown at bottom, and M28 (K28) killer viruses in S. cerevisiae. Saccharomyces cerevisiae virus L-A, also called L-A helper virus, is a member of the Totiviridae family of viruses found primarily in Saccharomyces cerevisiae. [3] Its discovery in the 1970s was the main starting point of research on yeast virology. [4]
In the peer-reviewed literature report, experimental results on function and interaction of yeast genes are extracted by high-quality manual curation and integrated within a well-developed database. The data are combined with quality high-throughput results and posted on Locus Summary pages which is a powerful query engine and rich genome browser.
Melting of DNA occurs within domain B2, induced by attachment of ARS binding factor 1 to B3. A1 and B1 domain binds with origin recognition complex. To identify these sequences, [1] yeast mutants unable to synthesize histidine were transformed with plasmids containing the His gene and random fragments of the yeast genome. If the genome fragment ...
The yeast genome is highly accessible to manipulation, hence it is an excellent model for genome engineering. The international Synthetic Yeast Genome Project (Sc2.0 or Saccharomyces cerevisiae version 2.0 ) aims to build an entirely designer, customizable, synthetic S. cerevisiae genome from scratch that is more stable than the wild type.
The degree of DNA bending is species and sequence dependent. For example, one study used the adenovirus TATA promoter sequence (5'-CGCTATAAAAGGGC-3') as a model binding sequence and found that human TBP binding to the TATA box induced a 97° bend toward the major groove while the yeast TBP protein only induced an 82° bend. [21]
The term viral protein refers to both the products of the genome of a virus and any host proteins incorporated into the viral particle. Viral proteins are grouped according to their functions, and groups of viral proteins include structural proteins , nonstructural proteins , regulatory proteins , and accessory proteins. [ 1 ]
The first genome to be sequenced was that of the virus φX174 in 1977; [4] the first genome sequence of a prokaryote (Haemophilus influenzae) was published in 1995; [5] the yeast (Saccharomyces cerevisiae) genome was the first eukaryotic genome to be sequenced in 1996. [6]
Examples of yeast expression vector in Pichia are the pPIC series of vectors, and these vectors use the AOX1 promoter which is inducible with methanol. [23] The plasmids may contain elements for insertion of foreign DNA into the yeast genome and signal sequence for the secretion of expressed protein.