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A latex fixation test, also called a latex agglutination assay or test (LA assay or test), is an assay used clinically in the identification and typing of many important microorganisms. These tests use the patient's antigen - antibody immune response.
Rapid diagnostic methods to detect cryptococcal antigen include latex agglutination testing, lateral flow immunochromatographic assay (LFA), or enzyme immunoassay (EIA). Polymerase chain reaction (PCR) has been used on tissue specimens, with PCR having a sensitivity of 82% and a specificity of 98% for cryptococcal infection.
Illustration of the effects of excess antigen and blocking antibodies on immunoassays. In an agglutination test, a person's serum (which contains antibodies) is added to a test tube, which contains a particular antigen. If the antibodies interact with the antigen to form immune complexes, called agglutination, then the test is interpreted as ...
Quellung is the German word for "swelling" and describes the microscopic appearance of pneumococcal or other bacterial capsules after their polysaccharide antigen has combined with a specific antibody. The antibody usually comes from serum taken from an immunized laboratory animal. As a result of this combination, and precipitation of the large ...
The agglutination between the antigen and the antibody is made with a specific antisera, which reacts with the antigen to produce a mass. The antigen O is tested with a bacterial suspension from an agar plate , whereas the antigen H is tested with a bacterial suspension from a broth culture.
Two years after finally being identified, the "Boy in the Box" case continues to haunt Philadelphia. The slain body of Joseph Augustus Zarelli, 4, was discovered in February 1957 in Philadelphia's ...
A pregnant woman is recovering in the hospital after she was stabbed multiple times by a pizza delivery driver over the size of her tip, according to reports. The incident happened on Sunday, Dec ...
The complement fixation test is an immunological medical test that can be used to detect the presence of either specific antibody or specific antigen in a patient's serum, based on whether complement fixation occurs. It was widely used to diagnose infections, particularly with microbes that are not easily detected by culture methods, and in ...