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Depending on the type of botulism considered, different tests for diagnosis may be indicated. Foodborne botulism: serum analysis for toxins by bioassay in mice should be done, as the demonstration of the toxins is diagnostic. [62] Wound botulism: isolation of C. botulinum from the wound site should be attempted, as growth of the bacteria is ...
Infant botulism results from the ingestion of the C. botulinum spores, and subsequent colonization of the small intestine. The infant gut may be colonized when the composition of the intestinal microflora (normal flora) is insufficient to competitively inhibit the growth of C. botulinum and levels of bile acids (which normally inhibit ...
Soil steam sterilization (soil steaming) is a farming technique that sterilizes soil with steam in open fields or greenhouses. Pests of plant cultures such as weeds, bacteria, fungi and viruses are killed through induced hot steam which causes vital cellular proteins to unfold. Biologically, the method is considered a partial disinfection.
Clostridium is a genus of anaerobic, Gram-positive bacteria.Species of Clostridium inhabit soils and the intestinal tracts of animals, including humans. [1] This genus includes several significant human pathogens, including the causative agents of botulism and tetanus.
Spores form a part of the life cycles of a diverse range of organisms such as many bacteria, plants, algae, fungi and some protozoa. In Saccharomyces cerevisiae (Kingdom Fungi), the set of early genes activating sporulation is induced by Ime1 (Inducer of Meiosis 1) and a regulator of middle genes is Ndt80p. [ 4 ]
While tetanus toxin is produced from Clostridium tetani, a spore forming bacteria found in soil, Tetanus is a paralytic disease that is global and commonly affects newborns as well as non-immunized individuals. Tetanus enters the body of organisms through wounds or skin breaks and can be found in manure, soil, and dust.
In microbiology, in the context of a sterilization procedure, the D-value or decimal reduction time (or decimal reduction dose) is the time (or dose of an antimicrobial drug) required, at a given condition (e.g. temperature) or set of conditions, to achieve a one-log reduction, that is, to kill 90% of relevant microorganisms. [1]
It has no known function for clostridia in the soil environment where they are normally encountered. It is also called spasmogenic toxin , tentoxilysin , tetanospasmin , or tetanus neurotoxin . The LD 50 of this toxin has been measured to be approximately 2.5–3 ng/kg, [ 2 ] [ 3 ] making it second only to the related botulinum toxin (LD 50 2 ...