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In microbiology, streaking is a technique used to isolate a pure strain from a single species of microorganism, often bacteria. Samples can then be taken from the resulting colonies and a microbiological culture can be grown on a new plate so that the organism can be identified, studied, or tested.
An inoculation loop (also called a smear loop, inoculation wand or microstreaker) is a simple tool used mainly by microbiologists to pick up and transfer a small sample of microorganisms called inoculum from a microbial culture, e.g. for streaking on a culture plate. [1] [2] This process is called inoculation.
Streaking on streak plates, fish tail inoculation of slant cultures and the inoculation of stab cultures can be done with the inoculation needle. [1] [7] Stab cultures specifically require the inoculation needle and is used to study cell motility, microbial oxygen requirements using Thioglycolate cultures, and the gelatin liquefaction of bacteria.
At some point during a successful "streak", the number of organisms deposited will be such that distinct individual colonies will grow in that area which may be removed for further culturing, using another sterile loop. Another way of plating organisms, next to streaking, on agar plates is the spot analysis. This type of analysis is often used ...
Some types of bacteria can only grow in the presence of certain additives. This can also be used when creating engineered strains of bacteria that contain an antibiotic-resistance gene. When the selected antibiotic is added to the agar, only bacterial cells containing the gene insert conferring resistance will be able to grow.
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Streaking yet again. Rotate the plate 180 degrees to get a proper streaking angle in the third quadrant. Be sure to cool the loops before streaking in quadrant four. Streaking in the center. Streak one last time beginning in quadrant four and into the center of the plate. Flame the loops. Incubate the plate for assigned time and appropriate ...
The vodka will kill the bacteria and doesn't leave a scent as it dries. Just make sure to spot test first. Clean windows and glass : Combine a cup of vodka, one-third cup of white vinegar and a ...