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The swarming capability of Proteus mirabilis is important because it is implicated in the pathogenesis of the bacteria and the swarming capability is associated with the bacteria's ability to express virulence factors [9] Proteus mirabilis has a very characteristic bulls-eye appearance on an agar plate due to the regular periodic cycling ...
Proteus is a genus of Gram-negative bacteria.It is a rod shaped, aerobic and motile bacteria, which is able to migrate across surfaces due its “swarming” characteristic in temperatures between 20 and 37 °C. [1]
One particular feature of this type of motility is the formation of dendritic fractal-like patterns formed by migrating swarms moving away from an initial location. Although the majority of species can produce tendrils when swarming, some species like Proteus mirabilis do form concentric circles motif instead of dendritic patterns. [19]
Among these are Pseudomonas aeruginosa, which has a grape-like scent; Staphylococcus aureus, which is said to smell like old socks; and Proteus mirabilis, whose scent is alternately described as putrid [1]: 168 or like chocolate cake. [7]: 124 Other distinctive features of colonial morphology include motility and the production of pigments.
Blood agar plates (BAPs) contain mammalian blood (usually sheep or horse), typically at a 5–10% concentration. BAPs are enriched, and differential media is used to isolate fastidious organisms and detect hemolytic activity. β-Hemolytic activity will show lysis and complete digestion of red blood cell contents surrounding a colony.
[1] [2] When, however, two different strains of Proteus are inoculated, the spreading films of growth fail to coalesce and remain separated by a narrow easily visible area. [2] The observation of this appearance, the Dienes phenomenon has been used to determine the identity or non-identity of strains in epidemiological studies .
They typically appear as medium to large-sized grey colonies on blood agar, although some can express pigments. Most have many flagella used to move about, but a few genera are nonmotile. Most members of Enterobacteriaceae have peritrichous, type I fimbriae involved in the adhesion of the bacterial cells to their hosts.
Agar plates are used as a canvas, while pigmented or fluorescent bacteria and yeasts represent paint. In order to preserve a piece of microbial art after a sufficient incubation, the microbe culture is sealed with epoxy. [2] Microbe species can be artistically chosen for their natural colours to form a palette.