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An example Gel documentation system, showing the results of gel electrophoresis on a connected monitor.. A gel doc, also known as a gel documentation system, gel image system or gel imager, refers to equipment widely used in molecular biology laboratories for the imaging and documentation of nucleic acid and protein suspended within polyacrylamide or agarose gels.
The integrity of RNA is a major concern for gene expression studies and traditionally has been evaluated using the 28S to 18S rRNA ratio, a method that has been shown to be inconsistent. [1] This inconsistency arises because subjective, human interpretation is necessary to compare the 28S and 18S gel images. The RIN algorithm was devised to ...
Unlike a spirit duplicator master, a hectograph master is not a mirror image. Thus, when using a spirit duplicator master with a hectograph, one writes on the back of the purple sheet, using it like carbon paper to produce an image on the white sheet, rather than writing on the front of the white sheet to produce a mirror image on its back.
Proteins are introduced into an immobilized pH gradient gel composed of polyacrylamide, starch, or agarose where a pH gradient has been established. Gels with large pores are usually used in this process to eliminate any "sieving" effects, or artifacts in the pI caused by differing migration rates for proteins of differing sizes.
1 2 3 A 1% agarose 'slab' gel under normal light, behind a perspex UV shield. Only the marker dyes can be seen: The gel with UV illumination, the ethidium bromide stained DNA glows orange: Digital photo of the gel. Lane 1. Commercial DNA Markers (1kbplus), Lane 2. empty, Lane 3. a PCR product of just over 500 bases, Lane 4.
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Photo 51 is an X-ray based fiber diffraction image of a paracrystalline gel composed of DNA fiber [1] taken by Raymond Gosling, [2] [3] a postgraduate student working under the supervision of Maurice Wilkins and Rosalind Franklin at King's College London, while working in Sir John Randall's group.