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The Kastle–Meyer test is a presumptive blood test, first described in 1903, in which the chemical indicator phenolphthalein is used to detect the possible presence of hemoglobin. It relies on the peroxidase -like activity of hemoglobin in blood to catalyze the oxidation of phenolphthalin (the colorless reduced form of phenolphthalein) into ...
Another common presumptive test is the Kastle-Meyer or Phenolphthalein test. This is a catalytic test that detects the heme group in blood that transports oxygen and carbon dioxide. [ 5 ] A sterile cotton swab is soaked in distilled water and applied to the area of suspected blood to pick up some of the sample. [ 5 ]
The Kastle Meyer (KM) reagent is actually already basic in pH -- it is when the reduced phenolphthalein in the reagent is oxidized to phenolphthalein by the presence of heme (or some other oxidizer) that it is the phenolphthalein you are familiar with, which indeed, will turn pink in the presence of its alkaline solvent.
The cell membranes may then be damaged if the horse is forced to continue work, which allows muscle enzymes and myoglobin to leak into the bloodstream. [1] This leads to the body building up a store of glycogen from converted carbohydrates in muscle cells. Glycogen is then depleted during work, and restocked when a horse rests.
Additionally, horses with a hind limb lameness will tend to reduce the degree of leg use. To do so, some horses will reduce the contraction time of the gluteals on the side of the lame leg, leading to a "hip roll" or "hip dip" and appearance that the hip drops a greater degree on the side of the lame leg. [10]
The Bavarian Warmblood is a horse breed of southern Germany that developed from an older Bavarian heavy warmblood breed called the Rottaler.Since mechanization in the mid-20th century, the Bavarian Regional Horse Breeders' Society has concentrated on producing a riding horse for the Olympic disciplines and recreational riding based on other European warmblood bloodlines.
Lameness is most commonly associated with injury to synovial joints, or those joints containing articular cartilage, a joint capsule, and a synovial membrane.Joint disease may affect the joint capsule and synovial membrane, articular cartilage, subchondral bone (the bone underneath the cartilage), menisci, or any ligaments associated with the joint.
As of 2009, the Percheron Horse Association of America had horses registered in all 50 states, and had nearly 3,000 members, with around 2,500 new horses being registered annually. [19] The French Société Hippique Percheronne de France (Percheron Horse Society of France) registered between 750 and 885 horses in each year between 2007 and 2010.