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Rufus was originally designed [5] as a modern open source replacement for the HP USB Disk Storage Format Tool for Windows, [6] which was primarily used to create DOS bootable USB flash drives. The first official release of Rufus, version 1.0.3 (earlier versions were internal/alpha only [ 7 ] ), was released on December 04, 2011, with originally ...
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Cas9 (or "CRISPR-associated protein 9") is an enzyme that uses CRISPR sequences as a guide to recognize and open up specific strands of DNA that are complementary to the CRISPR sequence. Cas9 enzymes together with CRISPR sequences form the basis of a technology known as CRISPR-Cas9 that can be used to edit genes within living organisms.
Universal USB Installer (UUI) is an open-source live Linux USB flash drive creation software. It allows users to create a bootable live USB flash drive using an ISO image from a supported Linux distribution, antivirus utility, system tool, or Microsoft Windows installer. The USB boot software can also be used to make Windows 8, 10, or 11 run ...
CRISPR RNA or crRNA is a RNA transcript from the CRISPR locus. [1] CRISPR-Cas (clustered, regularly interspaced short palindromic repeats - CRISPR associated systems) is an adaptive immune system found in bacteria and archaea to protect against mobile genetic elements , like viruses , plasmids , and transposons . [ 2 ]
See: Guide RNA, CRISPR. Complementary base pairing between the sgRNA and genomic DNA allows targeting of Cas9 or dCas9. A small guide RNA (sgRNA), or gRNA is an RNA with around 20 nucleotides used to direct Cas9 or dCas9 to their targets. gRNAs contain two major regions of importance for CRISPR systems: the scaffold and spacer regions.
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CRISPR/Cas9 edits rely on non-homologous end joining (NHEJ) or homology-directed repair (HDR) to fix DNA breaks, while the prime editing system employs DNA mismatch repair. This is an important feature of this technology given that DNA repair mechanisms such as NHEJ and HDR, generate unwanted, random insertions or deletions (INDELs).