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Ammonium sulfate is an inorganic salt with a high solubility that disassociates into ammonium (NH + 4) and sulfate (SO 2− 4) in aqueous solutions. [1] Ammonium sulfate is especially useful as a precipitant because it is highly soluble, stabilizes protein structure, has a relatively low density, is readily available, and is relatively inexpensive.
Calcium carbonate precipitates as a solid, leaving ammonium sulfate in the solution. (NH 4) 2 CO 3 + CaSO 4 → (NH 4) 2 SO 4 + CaCO 3. Ammonium sulfate occurs naturally as the rare mineral mascagnite in volcanic fumaroles and due to coal fires on some dumps. [14] Ammonium sulfate is a byproduct in the production of methyl methacrylate. [15]
The following chart shows the solubility of various ionic compounds in water at 1 atm pressure and room temperature (approx. 25 °C, 298.15 K). "Soluble" means the ionic compound doesn't precipitate, while "slightly soluble" and "insoluble" mean that a solid will precipitate; "slightly soluble" compounds like calcium sulfate may require heat to precipitate.
Salt compounds dissociate in aqueous solutions. This property is exploited in the process of salting out. When the salt concentration is increased, some of the water molecules are attracted by the salt ions, which decreases the number of water molecules available to interact with the charged part of the protein. [3]
The tables below provides information on the variation of solubility of different substances (mostly inorganic compounds) in water with temperature, at one atmosphere pressure. Units of solubility are given in grams of substance per 100 millilitres of water (g/100 ml), unless shown otherwise.
The amount of ammonia in ammonium salts can be estimated quantitatively by distillation of the salts with sodium (NaOH) or potassium hydroxide (KOH), the ammonia evolved being absorbed in a known volume of standard sulfuric acid and the excess of acid then determined volumetrically; or the ammonia may be absorbed in hydrochloric acid and the ...
Although most compounds are referred to by their IUPAC systematic names ... Ammonium sulfate – [NH 4] 2 SO 4 [53]
caesium hydrogen sulfate: 7789-16-4 Cs 2 C 2 O 4: caesium oxalate: 1068-63-9 Cs 2 CrO 4: caesium chromate: 13454-78-9 Cs 2 Cr 2 O 7: caesium dichromate: 13530-67-1 Cs 2 HPO 4: caesium hydrogen orthophosphate: Cs 2 MoO 4: caesium molybdate: 13597-64-3 Cs 2 O: caesium oxide: 20281-00-9 Cs 2 SO 3: caesium sulfite: Cs 2 SO 4: caesium sulfate: 10294 ...