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The fecal coliform assay is intended to be an indicator of fecal contamination; more specifically of E. coli which is an indicator microorganism for other pathogens that may be present in feces. Presence of fecal coliforms in water may not be directly harmful, and do not necessarily indicate the presence of feces. [1]
Fecal coliforms are more useful as indicators in recreational waters than total coliforms which include species that are naturally found in plants and soil; however, there are even some species of fecal coliforms that do not have a fecal origin, such as Klebsiella pneumoniae. Perhaps the biggest drawback to using coliforms as indicators is that ...
The solid medium is used to grow lactose-fermenting coliforms and utilizes a neutral red pH indicator. Pink colonies appear when lactose is fermented and are surrounded by bile that has precipitated out. To confirm if these colonies are coliforms, they are transferred to brilliant green lactose bile (BGLB) and incubated.
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Indicator bacteria themselves may not be pathogenic but their presence in waste may indicate the presence of other pathogens. [1] Similar to how there are various types of indicator organisms, there are also various types of indicator bacteria. The most common indicators are total coliforms, fecal coliforms, E. coli, and enterococci. [2]
Fecal coliform, at concentrations of 2,875 units/100 milliliters, was found in a pipe that drains into Laurel Creek, which winds through the city’s 26-acre Hunt Woods Park, where dogs bathe in ...
The presence of some coliforms indicate fecal contamination. The term "IMViC" is an acronym for each of these tests. "I" is for indole test; "M" is for methyl red test; "V" is for Voges-Proskauer test, and "C" is for citrate test. The lower case "i" is merely for "in" as the Citrate test requires coliform samples to be placed "in Citrate".
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