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Molecular biology techniques are common methods used in molecular biology, biochemistry, genetics and biophysics which generally involve manipulation and analysis of DNA, RNA, protein, and lipid Wikimedia Commons has media related to Molecular biology techniques .
Because molecular diagnostics methods can detect sensitive markers, these tests are less intrusive than a traditional biopsy. For example, because cell-free nucleic acids exist in human plasma , a simple blood sample can be enough to sample genetic information from tumours, transplants or an unborn fetus.
Biochemistry, Molecular biology: Gene knockout: Used to make one of an organism's genes inoperative ("knocked out" of the organism) Molecular biology, Genetics: Immunostaining: Used of an antibody-based method to detect a specific protein in a sample: Molecular biology, Biochemistry: Intracellular recording: Used to measure the voltage across a ...
Nucleic acid sequence-based amplification, commonly referred to as NASBA, is a method in molecular biology which is used to produce multiple copies of single stranded RNA. [1] NASBA is a two-step process that takes RNA and anneals specially designed primers, then utilizes an enzyme cocktail to amplify it. [2]
Molecular imprinting is a technique to create template-shaped cavities in polymer matrices with predetermined selectivity and high affinity. [1] This technique is based on the system used by enzymes for substrate recognition, which is called the "lock and key" model. The active binding site of an enzyme has a shape specific to a substrate.
Methods in Molecular Biology is a book series published by Humana Press (an imprint of Springer Science+Business Media) that covers molecular biology research methods and protocols. The book series was introduced by series editor John M. Walker in 1983 and provides step-by-step instructions for carrying out experiments in a research lab. [1]
Several molecular typing schemes have been proposed to determine the relatedness of pathogens such as pulsed-field gel electrophoresis , ribotyping, and PCR-based fingerprinting. But these DNA banding-based subtyping methods do not provide meaningful evolutionary analyses.
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related to: molecular methods in microbiology pdf format template powerpoint ppt