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A microbiological culture, or microbial culture, is a method of multiplying microbial organisms by letting them reproduce in predetermined culture medium under controlled laboratory conditions. Microbial cultures are foundational and basic diagnostic methods used as research tools in molecular biology .
The Winogradsky column is a simple device for culturing a large diversity of microorganisms. Invented in the 1880s by Sergei Winogradsky , the device is a column of pond mud and water mixed with a carbon source such as newspaper (containing cellulose ), blackened marshmallows or egg-shells (containing calcium carbonate ), and a sulfur source ...
The Isolation chip (or ichip) is a method of culturing bacteria. Using regular methods, 99% of bacterial species are not able to be cultured as they do not grow in conditions made in a laboratory, a problem called the "Great Plate Count Anomaly". [1] The ichip instead cultures bacterial species within its soil environment.
In microbiology, a colony-forming unit (CFU, cfu or Cfu) is a unit which estimates the number of microbial cells (bacteria, fungi, viruses etc.) in a sample that are viable, able to multiply via binary fission under the controlled conditions. Counting with colony-forming units requires culturing the microbes and counts only viable cells, in ...
Gram-negative bacteria will stain a pink color due to the thin layer of peptidoglycan. If a bacteria stains purple, due to the thick layer of peptidoglycan, the bacteria is a gram-positive bacteria. [4] In clinical microbiology numerous other staining techniques for particular organisms are used (acid fast bacterial stain for mycobacteria).
Sabouraud agar is used to culture fungi and has a low pH that inhibits the growth of most bacteria; it also contains the antibiotic gentamicin to specifically inhibit the growth of Gram-negative bacteria. Hay infusion agar is specific for the culturing of slime moulds (which are not fungi). Potato dextrose agar is used to culture certain types ...
for viral culture detection Hungate Anaerobic tubes: for culturing of anaerobic microbes Incubator: used for bacterial or fungal cultures Inoculation loop: used to inoculate test samples into culture media for bacterial or fungal cultures, antibiograms, etc. Sterilized by passing through a blue flame. Laminar flow cabinet: used to work aseptic
Plate count agar (PCA), also called standard methods agar (SMA), is a microbiological growth medium commonly used to assess or to monitor "total" or viable bacterial growth of a sample. PCA is not a selective medium. The total number of living aerobic bacteria can be