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Openclipart, also called Open Clip Art Library, is an online media repository of free-content vector clip art.The project hosts over 160,000 free graphics and has billed itself as "the largest community of artists making the best free original clipart for you to use for absolutely any reason".
Cosmids can contain 37 to 52 (normally 45) kb of DNA, limits based on the normal bacteriophage packaging size. They can replicate as plasmids if they have a suitable origin of replication (ori): for example SV40 ori in mammalian cells, ColE1 ori for double-stranded DNA replication, or f1 ori for single-stranded DNA replication in prokaryotes .
The first step in Gateway cloning is the preparation of a Gateway Entry clone. There are a few different ways to make entry clone. Gateway attB1 and attB2 sequences are added to the 5' and 3' end of a gene fragment, respectively, using gene-specific PCR primers and PCR amplification. The PCR amplification products are then mixed with a propriet
This may be a multiple cloning site (MCS) or polylinker, which contains many unique restriction sites. The restriction sites in the MCS are first cleaved by restriction enzymes, then a PCR-amplified target gene also digested with the same enzymes is ligated into the vectors using DNA ligase. The target DNA sequence can be inserted into the ...
This adapter can be used to convert the cohesive end produced by Bam Hl to one produced by Eco Rl or vice versa. One of its applications is ligating cDNA into a plasmid [ 3 ] or other vectors instead of using Terminal deoxynucleotide Transferase enzyme to add poly A to the cDNA fragment.
New York to get 'state-of-the-art' detection system. Also Sunday, New York Gov. Kathy Hochul said the federal government will deploy a new drone detection system in her state. Drones forced ...
Nicotine Replacement Therapy. Among your NRT options are nicotine pouches and patches. Pouches directly supply low doses of nicotine through oral absorption.
The recognition sites required for pN action are called nut (for N utilization). The sites responsible for determining leftward and rightward antitermination are described as nut L and nut G, respectively. When pN recognizes the nut site, it forms a persistent antitermination complex in cooperation with a number of E. coli host proteins. These ...