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The hemolysis of some weakly beta-hemolytic organisms is enhanced when streaked close to certain beta hemolytic strains of Staphylococcus aureus. This phenomenon is the mechanism behind the CAMP test, [2] a test that was historically used for the identification of Streptococcus agalactiae and Listeria monocytogenes. [3]
CLED agar (cystine–lactose–electrolyte-deficient agar or medium) is a valuable non-inhibitory growth medium used in the isolation and differentiation of urinary microbes. It contains cystine and lactose and is electrolyte -deficient; the latter trait prevents the swarming of Proteus species.
Vogel–Johnson agar is a type of agar growth medium selective for coagulase-positive staphylococci. It is used to isolate Staphylococcus aureus from clinical specimens and food. It was first described by Vogel and Johnson, who modified the Tellurite Glycine Agar recipe by Zebovitz et al. by doubling the mannitol concentration to 1% (w/v) and ...
CLED agar – cysteine, lactose, electrolyte-deficient agar is used to isolate and differentiate urinary tract bacteria, since it inhibits Proteus species swarming and can distinguish between lactose fermenters and nonfermenters. Granada medium is used to isolate and differentiate group B Streptococcus, Streptococcus agalactiae from clinical ...
Staphylococcus aureus is a gram-positive spherically shaped bacterium, a member of the Bacillota, and is a usual member of the microbiota of the body, frequently found in the upper respiratory tract and on the skin. It is often positive for catalase and nitrate reduction and is a facultative anaerobe, meaning that it can grow without oxygen. [1]
An MSA plate with Micrococcus sp. (1), Staphylococcus epidermidis (2) and S. aureus colonies (3). Mannitol salt agar or MSA is a commonly used selective and differential growth medium in microbiology. It encourages the growth of a group of certain bacteria while inhibiting the growth of others. [1]
Staphylococcus growth in Baird-Parker (BP) agar.. Baird-Parker agar is a type of agar used for the selective isolation of gram-positive Staphylococci species. [1] It contains lithium chloride and tellurite to inhibit the growth of alternative microbial flora, while the included pyruvate and glycine promote the growth of Staphylococci. [1]
Staphylococcus species are facultative anaerobes (capable of growth both aerobically and anaerobically). [15] All species grow in the presence of bile salts. All strains of Staphylococcus aureus were once thought to be coagulase-positive, but this has since been disproven. [16] [17] [18] Growth can also occur in a 6.5% NaCl solution. [15]