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Escherichia coli (/ ... The first complete DNA sequence of an E. coli genome (laboratory strain K-12 derivative MG1655) was published in 1997.
Since it is a DNA-dependent ATPase, RecA contains an additional site for binding and hydrolyzing ATP. RecA associates more tightly with DNA when it has ATP bound than when it has ADP bound. [14] Homologous recombination events mediated by RecA can occur in Escherichia coli during the period after DNA replication when sister loci remain close.
In Escherichia coli, different classes of DNA-damaging agents can initiate the SOS response, as described above. Taking advantage of an operon fusion placing the lac operon (responsible for producing beta-galactosidase, a protein which degrades lactose) under the control of an SOS-related protein, a simple colorimetric assay for genotoxicity is ...
E. coli colonies containing the fluorescent pGLO plasmid. Escherichia coli (/ ˌ ɛ ʃ ɪ ˈ r ɪ k i ə ˈ k oʊ l aɪ /; commonly abbreviated E. coli) is a Gram-negative gammaproteobacterium commonly found in the lower intestine of warm-blooded organisms (endotherms). The descendants of two isolates, K-12 and B strain, are used routinely in ...
The phi X 174 (or ΦX174) bacteriophage is a single-stranded DNA virus that infects Escherichia coli. This virus was isolated in 1935 by Nicolas Bulgakov [1] in Félix d'Hérelle's laboratory at the Pasteur Institute, from samples collected in Paris sewers. Its characterization and the study of its replication mechanism were carried out from ...
dnaQ is the gene encoding the ε subunit of DNA polymerase III in Escherichia coli. [1] The ε subunit is one of three core proteins in the DNA polymerase complex. It functions as a 3’→5’ DNA directed proofreading exonuclease that removes incorrectly incorporated bases during replication. [2] dnaQ may also be referred to as mutD. [3]
DH5-Alpha Cells are E. coli cells engineered by American biologist Douglas Hanahan to maximize transformation efficiency. They are defined by three [1] mutations: recA1, endA1 which help plasmid insertion and lacZΔM15 which enables blue white screening.
In the bacterium Escherichia coli, RecD protein is part of the well studied RecBCD complex that is necessary for recombinational DNA repair (as described above). In the bacterium Bacillus subtilis , RecD2 protein has a role as a modulator of replication restart and also a modulator of the RecA recombinase. [ 21 ]