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  2. Transcription (biology) - Wikipedia

    en.wikipedia.org/wiki/Transcription_(biology)

    Both DNA and RNA are nucleic acids, which use base pairs of nucleotides as a complementary language. During transcription, a DNA sequence is read by an RNA polymerase, which produces a complementary, antiparallel RNA strand called a primary transcript. In virology, the term transcription is used when referring to mRNA synthesis from a viral RNA ...

  3. Central dogma of molecular biology - Wikipedia

    en.wikipedia.org/wiki/Central_dogma_of_molecular...

    A second version of the central dogma is popular but incorrect. This is the simplistic DNARNAprotein pathway published by James Watson in the first edition of The Molecular Biology of the Gene (1965). Watson's version differs from Crick's because Watson describes a two-step (DNARNA and RNAprotein) process as the central ...

  4. DNA and RNA codon tables - Wikipedia

    en.wikipedia.org/wiki/DNA_and_RNA_codon_tables

    A codon table can be used to translate a genetic code into a sequence of amino acids. [1] [2] The standard genetic code is traditionally represented as an RNA codon table, because when proteins are made in a cell by ribosomes, it is messenger RNA (mRNA) that directs protein synthesis. [2] [3] The mRNA sequence is determined by the sequence of ...

  5. Protein production - Wikipedia

    en.wikipedia.org/wiki/Protein_production

    For example, a DNA sequence for a protein of interest could be cloned or subcloned into a high copy-number plasmid containing the lac (often LacUV5) promoter, which is then transformed into the bacterium E. coli. Addition of IPTG (a lactose analog) activates the lac promoter and causes the bacteria to express the protein of interest. [2]

  6. Nucleic acid sequence - Wikipedia

    en.wikipedia.org/wiki/Nucleic_acid_sequence

    A nucleic acid sequence is a succession of bases within the nucleotides forming alleles within a DNA (using GACT) or RNA (GACU) molecule. This succession is denoted by a series of a set of five different letters that indicate the order of the nucleotides. By convention, sequences are usually presented from the 5' end to the 3' end.

  7. Adaptor hypothesis - Wikipedia

    en.wikipedia.org/wiki/Adaptor_hypothesis

    Watson and Alexander Rich discussed in the PNAS, saying, "We shall not be able to check a structural relationship between RNA and protein synthesis or between RNA and DNA until we know the structure of RNA." [5] Evidences had been accumulating since the 1940s that protein synthesis occurs simultaneously with increased level of RNA in the cytoplasm.

  8. NASBA (molecular biology) - Wikipedia

    en.wikipedia.org/wiki/NASBA_(molecular_biology)

    Nucleic acid sequence-based amplification, commonly referred to as NASBA, is a method in molecular biology which is used to produce multiple copies of single stranded RNA. [1] NASBA is a two-step process that takes RNA and anneals specially designed primers, then utilizes an enzyme cocktail to amplify it.

  9. Systematic evolution of ligands by exponential enrichment

    en.wikipedia.org/wiki/Systematic_evolution_of...

    RNA and DNA secondary structure prediction by dynamic programming algorithms such as RNAfold [36] and by machine learning models such as SPOT-RNA, [37] MXfold2 [38] provides the opportunity to assess the ability of sequences in the primary library to fold into complex structures, allowing for the selection of only the most promising sequences ...

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