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The jar, about 20 by 12.5 inches (510 mm × 320 mm) is made of metal. Its parts are as follows: The body made up of metal (airtight) The lid, also metal can be placed in an airtight fashion; A screw going through a curved metal strip to secure and hold the lid in place; A thermometer to measuring the internal temperature
Candle jar: historically used for anaerobiosis; a lit candle was placed in as air-tight jar such that when it went out it would be because it used up all the available oxygen: Castaneda's medium / Castaneda's bottle: used for simultaneous solid and liquid cultures in many bottles Centrifuge: to separate supernatant & pellet Cragie tube: see ...
They began to experiment to find the ideal environment for maintaining cell culture stocks. These early incubators were simply made up of bell jars that contained a single lit candle. Cultures were placed near the flame on the underside of the jar's lid, and the entire jar was placed in a dry, heated oven. Incubator invented by Hess
A vacuum bell jar is placed on a base which is vented to a hose fitting, that can be connected via a hose to a vacuum pump. A vacuum is formed by pumping the air out of the bell jar. The lower edge of a vacuum bell jar forms a flange of heavy glass, ground smooth on the bottom for better contact. The base of the jar is equally heavy and flattened.
The sealed anaerobic jar is then incubated at a desired temperature to allow growth of the bacteria. In the above figure for example, the incubation occurred at 100 °F (38 °C). A colorless indicator strip provides proof that the anaerobic conditions were met and the agar plates can now be observed for bacterial growth.
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