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Cell isolation is the process of separating individual living cells from a solid block of tissue or cell suspension. While some types of cell naturally exist in a separated form (for example blood cells ), other cell types that are found in solid tissue require specific techniques to separate them into individual cells.
Primary cell culture is the ex vivo culture of cells freshly obtained from a multicellular organism, as opposed to the culture of immortalized cell lines.In general, primary cell cultures are considered more representative of in vivo tissues than cell lines, and this is recognized legally in some countries such as the UK (Human Tissue Act 2004). [1]
These primary cells can then be further expanded and transferred into fresh dishes through micropropagation. Explant culture can also refer to the culturing of the tissue pieces themselves, where cells are left in their surrounding extracellular matrix to more accurately mimic the in vivo environment e.g. cartilage explant culture, [ 3 ] or ...
Besides the culture of well-established immortalised cell lines, cells from primary explants of a plethora of organisms can be cultured for a limited period of time before senescence occurs (see Hayflick's limit). Cultured primary cells have been extensively used in research, as is the case of fish keratocytes in cell migration studies. [88 ...
Currently six primary isolation/collection technologies exist using a microscope and device for cell isolation. Four of these typically use an ultraviolet pulsed laser (355 nm) for the cutting of the tissues directly or the membranes/film, and sometimes in combination with an IR laser responsible for heating/melting a sticky polymer for ...
(3) This technique of separation does not only consist of separation of cells within the blood, but can also be used for techniques of separation from primary tumors and in metastases research, through separation into component parts, creating a singular-cell delay, then allowing the suitable antibody to label the cell.
Black polyethylene microspheres can have magnetic or conductive functionality, and have uses in electronic devices, EMI shielding, and microscopy techniques. [ 6 ] [ 7 ] Fluorescent polyethylene microspheres are commonly used to run blind tests on laboratory and industrial processes, in order to develop proper methods and minimize cross ...
Primary containment requires using proper storage containers, good microbiological technique, and the use of appropriate safety equipment such as biological safety cabinets. Secondary containment is the protection of the environment external to the laboratory from exposure to infectious materials and is provided by a combination of facility ...