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Protein detection can monitor soybean protein labeling system in processed foods to protect consumers in a reliable way. [8] The labeling for soybean protein declaimed by protein detection has indicated to be the most important solution. [8] Detailed labeling description for the soybean ingredients in refined foods is required to protect the ...
Protein labeling use a short tag to minimize disruption of protein folding and function. Transition metals are used to link specific residues in the tags to site-specific targets such as the N-termini, C-termini, or internal sites within the protein. Examples of tags used for protein labeling include biarsenical tags, Histidine tags, and FLAG tags.
A typical workflow of a peptide mass fingerprinting experiment. Peptide mass fingerprinting (PMF), also known as protein fingerprinting, is an analytical technique for protein identification in which the unknown protein of interest is first cleaved into smaller peptides, whose absolute masses can be accurately measured with a mass spectrometer such as MALDI-TOF or ESI-TOF. [1]
An evaluation of the western blot's ability to detect antibodies against F. tularensis revealed that its sensitivity is almost 100% and the specificity is 99.6%. [12] Some forms of Lyme disease testing employ western blotting. [13] A western blot can also be used as a confirmatory test for Hepatitis B infection and HSV-2 (Herpes Type 2) infection.
Robotic preparation of MALDI mass spectrometry samples on a sample carrier. Proteomics is the large-scale study of proteins. [1] [2] Proteins are vital macromolecules of all living organisms, with many functions such as the formation of structural fibers of muscle tissue, enzymatic digestion of food, or synthesis and replication of DNA.
The characteristic color of a positive biuret test. In chemistry, the biuret test (IPA: / ˌ b aɪ j ə ˈ r ɛ t /, / ˈ b aɪ j ə ˌ r ɛ t / [1]), also known as Piotrowski's test, is a chemical test used for detecting the presence of at least two peptide bonds in a molecule.
DNA polymerase enzymes do not suffer the same inhibition and is also readily multiplexable [12] and has been multiplexed up to 384 proteins. [13] PEA performance is temperature sensitive as it is a DNA hybridization-based reaction. So the use of hyper-thermostable polymerases with no activity at room temperature supports bench top reaction ...
Recognition of many proteins' ability to spontaneously assemble into functional complexes as well as the ability of protein fragments to assemble as a consequence of the spontaneous functional complex assembly of interaction partners to which they are fused was later reported for ubiquitin fragments in yeast protein interactions. [6]