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Giemsa's solution is a mixture of methylene blue, eosin, and Azure B.The stain is usually prepared from commercially available Giemsa powder. A thin film of the specimen on a microscope slide is fixed in pure methanol for 30 seconds, by immersing it or by putting a few drops of methanol on the slide.
Routine analysis of blood in medical laboratories is usually performed on blood films stained with Romanowsky stains such as Wright's stain, Giemsa stain, or Diff-Quik. Wright-Giemsa combination stain is also a popular choice. These stains allow for the detection of white blood cell, red blood cell, and platelet abnormalities.
Lymphatic filariasis is a human disease caused by parasitic worms known as filarial worms. [2] [3] Usually acquired in childhood, it is a leading cause of permanent disability worldwide, impacting over a hundred million people and manifesting itself in a variety of severe clinical pathologies [6] [7] While most cases have no symptoms, some people develop a syndrome called elephantiasis, which ...
The most influential 1904 work. Giemsa was born in Hamburg to Gustav, a mining official and Franziska. He studied pharmacy and mineralogy at the University of Leipzig (1892-94), and then worked between 1895 and 1898 as a pharmacist at the government hospital in Dar-es-Salaam, German East Africa.
A skin scraping can be prepared, and stained with Wright's stain. Many intracellular and extracellular yeast cells with crosswalls are suggestive of T. marneffei infection. Smears from bone marrow aspirates may also be taken; this is regarded as the most sensitive method. These samples can be stained with the Giemsa stain.
Brugia is a genus for a group of small roundworms. They are among roundworms that cause the parasitic disease filariasis. [1] Specifically, of the three species known, Brugia malayi and Brugia timori cause lymphatic filariasis in humans; and Brugia pahangi and Brugia patei infect domestic cats, dogs and other animals.
G-banding, G banding or Giemsa banding is a technique used in cytogenetics to produce a visible karyotype by staining condensed chromosomes. It is the most common chromosome banding method. [ 1 ] It is useful for identifying genetic diseases (mainly chromosomal abnormalities ) through the photographic representation of the entire chromosome ...
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