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  2. Genetic engineering - Wikipedia

    en.wikipedia.org/wiki/Genetic_engineering

    Genetic engineering, also called genetic modification or genetic manipulation, is the modification and manipulation of an organism's genes using technology. It is a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms .

  3. Genetic engineering techniques - Wikipedia

    en.wikipedia.org/wiki/Genetic_engineering_techniques

    Genetic engineering techniques allow the modification of animal and plant genomes. Techniques have been devised to insert, delete, and modify DNA at multiple levels, ranging from a specific base pair in a specific gene to entire genes. There are a number of steps that are followed before a genetically modified organism (GMO) is created.

  4. DNA shuffling - Wikipedia

    en.wikipedia.org/wiki/DNA_shuffling

    In StEP, brief cycles of primer annealing to a template and extension by polymerase are employed to generate full-length sequences. [31] [32] The main advantages of StEP are the simplicity of the method and the lack of fragment purification. [7] [13] The disadvantages of StEP include that it is time consuming and requires sequence homology. [7 ...

  5. Biotechnology - Wikipedia

    en.wikipedia.org/wiki/Biotechnology

    Genetic testing identifies changes in chromosomes, genes, or proteins. [51] Most of the time, testing is used to find changes that are associated with inherited disorders. The results of a genetic test can confirm or rule out a suspected genetic condition or help determine a person's chance of developing or passing on a genetic disorder. As of ...

  6. Gene targeting - Wikipedia

    en.wikipedia.org/wiki/Gene_targeting

    A Venn Diagram to show the relationship between three types of 'Genetic engineering'; Genetic Modification, Gene Targeting and Genome Editing. The relationship between gene targeting, gene editing and genetic modification is outlined in the Venn diagram below. It displays how 'Genetic engineering' encompasses all 3 of these techniques.

  7. Modifications (genetics) - Wikipedia

    en.wikipedia.org/wiki/Modifications_(genetics)

    Genetic engineering is a type of intentional genetic modification, which uses biotechnology to alter an organism's genome. [ citation needed ] According to World Health Organization (WHO), genetically modified organisms are defined as "Organisms (i.e. plants, animals or microorganisms) in which the genetic material (DNA) has been altered in a ...

  8. Genetic transformation - Wikipedia

    en.wikipedia.org/wiki/Genetic_transformation

    This process of the second bacterial cell taking up new genetic material is called transformation. In molecular biology and genetics, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material from its surroundings through the cell membrane(s).

  9. Gene knockout - Wikipedia

    en.wikipedia.org/wiki/Gene_knockout

    CRISPR-based gene knockout is a powerful tool for understanding the genetic basis of disease and for developing new therapies. It is important to note that CRISPR-based gene knockout, like any genetic engineering technique, has the potential to produce unintended or harmful effects on the organism, so it should be used with caution.