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The frozen section procedure as practiced today in medical laboratories is based on the description by Dr Louis B. Wilson in 1905. Wilson developed the technique from earlier reports at the request of Dr William Mayo, surgeon and one of the founders of the Mayo Clinic [3] Earlier reports by Dr Thomas S. Cullen at Johns Hopkins Hospital in Baltimore also involved frozen section, but only after ...
Cryopreservation or cryoconservation is a process where biological material - cells, tissues, or organs - are frozen to preserve the material for an extended period of time. [1] At low temperatures (typically −80 °C (−112 °F) or −196 °C (−321 °F) using liquid nitrogen ) any cell metabolism which might cause damage to the biological ...
The second method of histology processing is called frozen section processing. This is a highly technical scientific method performed by a trained histoscientist. In this method, the tissue is frozen and sliced thinly using a microtome mounted in a below-freezing refrigeration device called the cryostat. The thin frozen sections are mounted on ...
ICSI sperm injection into oocyte. Oocyte cryopreservation is a procedure to preserve a woman's eggs ().The technique is often used to delay pregnancy.At the time pregnancy is desired, the eggs can be thawed, fertilized, and transferred to the uterus as embryos.
Frozen section procedure: water-rich tissues are hardened by freezing and cut in the frozen state with a freezing microtome or microtome-cryostat; sections are stained and examined with a light microscope. This technique is much faster than traditional histology (5 minutes vs 16 hours) and is used in conjunction with medical procedures to ...
Frozen section procedure: tissue embedded in optimal cutting temperature compound, mounted on a chuck in a cryostat and ready for section production. Optimal cutting temperature (OCT) compound is used to embed tissue samples prior to frozen sectioning on a microtome-cryostat. This process is undertaken so as to mount slices (sections) of a ...
Modern frozen section method. Frozen section histology does not give the added margin of safety by the cytotoxic Mohs paste, [14] originally used by Mohs. This paste might have destroyed any residual cancer cells not detected by the pathologist. Missing epidermal margins. Ideally, the Mohs section should include 100% of the epidermal margin ...
In current state of the art, early embryos having undergone cryopreservation implant at the same rate as equivalent fresh counterparts. [2] The outcome from using cryopreserved embryos has uniformly been positive with no increase in birth defects or development abnormalities, [3] [8] also between fresh versus frozen eggs used for intracytoplasmic sperm injection (ICSI). [9]