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A Soxhlet extractor is a piece of laboratory apparatus [1] invented in 1879 by Franz von Soxhlet. [2] It was originally designed for the extraction of a lipid from a solid material. Typically, Soxhlet extraction is used when the desired compound has a limited solubility in a solvent , and the impurity is insoluble in that solvent.
The history of scientific method considers changes in the methodology of scientific inquiry, not the history of science itself. The development of rules for scientific reasoning has not been straightforward; scientific method has been the subject of intense and recurring debate throughout the history of science, and eminent natural philosophers and scientists have argued for the primacy of ...
Illustrations are also downloadable as PowerPoint slides. These files can be easily copied and pasted into any existing PowerPoint presentation or can serve as a starting point for a new presentation. These slides are designed to provide lecturers with an easy-to-use and convenient source of illustrations for genetic terms and concepts.
A scientific theory is an explanation of an aspect of the natural world and universe that can be or that has been repeatedly tested and has corroborating evidence in accordance with the scientific method, using accepted protocols of observation, measurement, and evaluation of results.
Plasmid miniprep. 0.8% agarose gel ethidium bromide-stained.. A plasmid preparation is a method of DNA extraction and purification for plasmid DNA.It is an important step in many molecular biology experiments and is essential for the successful use of plasmids in research and biotechnology.
The second method is primary explant culture, in which fragments derived from animal tissue are attached to a surface using an extracellular matrix component (ECM), such as collagen or a plasma clot. This culture is known as a primary explant, and migrating cells are known as outgrowth.
The method is named after the British biologist Edwin Southern, who first published it in 1975. [3] Other blotting methods (i.e., western blot , [ 4 ] northern blot , eastern blot , southwestern blot ) that employ similar principles, but using RNA or protein, have later been named for compass directions as a sort of pun from Southern's name.
Cross-linking and immunoprecipitation (CLIP, or CLIP-seq) is a method used in molecular biology that combines UV crosslinking with immunoprecipitation in order to identify RNA binding sites of proteins on a transcriptome-wide scale, thereby increasing our understanding of post-transcriptional regulatory networks.