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A Soxhlet extractor is a piece of laboratory apparatus [1] invented in 1879 by Franz von Soxhlet. [2] It was originally designed for the extraction of a lipid from a solid material. Typically, Soxhlet extraction is used when the desired compound has a limited solubility in a solvent , and the impurity is insoluble in that solvent.
Microtechnique is an aggregate of methods used to prepare micro-objects for studying. [1] It is currently being employed in many fields in life science. Two well-known branches of microtechnique are botanical (plant) microtechnique and zoological (animal) microtechnique.
A microscope slide (top) and a cover slip (bottom) A microscope slide is a thin flat piece of glass, typically 75 by 26 mm (3 by 1 inches) and about 1 mm thick, used to hold objects for examination under a microscope. Typically the object is mounted (secured) on the slide, and then both are inserted together in the microscope for viewing. This ...
The slide is left to air dry, after which the blood is fixed to the slide by immersing it briefly in methanol. The fixative is essential for good staining and presentation of cellular detail. After fixation, the slide is stained to distinguish the cells from each other. [citation needed]
This list of research methods in biology is an index to articles about research methodologies used in various branches of biology. ... Hardy–Weinberg principle:
The slides are then dehydrated in ethanol, and the probe mixture is added. The sample DNA and the probe DNA are then co-denatured using a heated plate and allowed to re-anneal for at least 4 hours. The slides are then washed to remove the excess unbound probe, and counterstained with 4',6-Diamidino-2-phenylindole or propidium iodide.
As a simple alternative for mortar and pestle, frosted glass slides are used for homogenizing small and soft tissue specimens. Tissue specimen is homogenized in a small volume of appropriate buffer between corner areas of two frosted glass slides by circular movement; then homogenate is collected by a pipette.
In addition, in-situ hybridization on tissue sections require that tissue slices be very thin, usually 3 μm to 7 μm in thickness. Common methods of preparing tissue sections for in-situ hybridization processing include cutting specimens with a cryostat or a Compresstome tissue slicer.