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Besides BSA, fetal bovine serum is a rich source of growth and attachment factors, lipids, hormones, nutrients and electrolytes necessary to support cell growth in culture. It is typically added to basal cell culture medium, such as DMEM or RPMI , at a 5–10% concentration.
Normally, the medium contains no proteins or growth factors, so it is commonly supplemented with 10% fetal bovine serum. Properly supplemented with serum or an adequate serum replacement, RPMI 1640 allows the cultivation of many cell types, especially human lymphocytes, Jurkat cells, HeLa cells, bone marrow cells, hybridomas and carcinomas.
They are a suspension cell line and are best cultured in Eagle's minimal essential medium with 10% Fetal bovine serum, 0.01 units/ml of penicillin and 0.1 mg/ml streptomycin incubated in 37 °C + 5% CO 2 + 90% relative humidity with a cell density of 5x10 5 cell per 15 ml.
KBM-7 cells grow in suspension and are maintained in Iscove's Modified Dulbecco's Medium (IMDM) supplemented with 10% fetal bovine serum. They divide approximately every 24 hours. They divide approximately every 24 hours.
LN95 cells were derived from LNCaP cultured in RPMI-1640 (without phenol red) with 10% charcoal stripped fetal bovine serum. [7] LN95 cells differ from parental LNCaP cells morphologically, with pronounced dendritic extensions, and molecularly, with Androgen receptor variant expression similar to that of AR-V7 High VCaP cells. [8]
The base medium for this cell line is RPMI-1640 Medium with a fetal bovine serum in a final concentration of 10%. 4T1 cells should not be allowed to become 100% confluent. Subculturing at 80% confluence is recommended.
Though HT-29 cells can proliferate in cell culture lacking growth factors with a doubling time of around 4 days, the doubling time can be reduced to one day with added fetal bovine serum. [2] The cells have high glucose consumption, and in standard medium containing 25 mM glucose and 10% serum, remain undifferentiated. [1]
The most common growing cocktail used is a 1:1 mixture of DMEM and Ham's F12 medium and 10% supplemental fetal bovine serum. The DMEM usually contains 3.7 g/L sodium bicarbonate, 2 mM L-Glutamine, 1 mM sodium pyruvate and 0.1 mM nonessential amino acids. [8] The cells are always grown at 37 degrees Celsius with 95% air and 5% carbon dioxide. It ...
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