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The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century.
The pupil function or aperture function describes how a light wave is affected upon transmission through an optical imaging system such as a camera, microscope, or the human eye. More specifically, it is a complex function of the position in the pupil [ 1 ] or aperture (often an iris ) that indicates the relative change in amplitude and phase ...
Modern microscopes, known as compound microscopes have many lenses in them (typically four) to optimize the functionality and enhance image stability. [111] A slightly different variety of microscope, the comparison microscope, looks at side-by-side images to produce a stereoscopic binocular view that appears three dimensional when used by ...
When light waves travel through a medium other than a vacuum, interaction with the medium causes the wave amplitude and phase to change in a manner dependent on properties of the medium. Changes in amplitude (brightness) arise from the scattering and absorption of light, which is often wavelength-dependent and may give rise to colors.
As the combined image keeps both amplitude and phase information, the interferometric microscopy can be especially efficient for the phase objects, [3] allowing detection of light variations of index of refraction, which cause the phase shift or the light passing through for a small fraction of a radian.
An optical instrument is a device that processes light waves (or photons), either to enhance an image for viewing or to analyze and determine their characteristic properties. Common examples include periscopes, microscopes, telescopes, and cameras. [1] [2]
The single lens with its attachments, or the system of lenses and imaging equipment, along with the appropriate lighting equipment, sample stage, and support, makes up the basic light microscope. The most recent development is the digital microscope, which uses a CCD camera to focus on the exhibit of interest. The image is shown on a computer ...
While this term is often also used to refer to high resolution scanning transmission electron microscopy, mostly in high angle annular dark field mode, this article describes mainly the imaging of an object by recording the two-dimensional spatial wave amplitude distribution in the image plane, similar to a "classic" light microscope.